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Title:

????? Proteomics

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Title: Proteomics Author: zczhang Last modified by: zczhang Created Date: 4/23/2008 12:52:20 AM Document presentation format: – PowerPoint PPT presentation

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Title: ????? Proteomics


1
????? Proteomics
  • ???
  • ???????????

Nature, 2006,439168
2
????????
  • 1995?,???????????,???????????????????????,????????
    ??,??????,??????????,?????????????????,???????????
    ??????
  • 2001?,Nature,Science????? And now for the
    proteome Proteomics in genomeland

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??????
  • ????????????????????,???SDS-PAGE???????????,??????
    ???????????????????????

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?????????????(MALDI)???
  • ??????????????,?????????????? 1987?,?????-????????
    ???,???????????????????????????????,?????????????R
    apid Communications in Mass Spectrometry??????????
    ?????????????(Maldi)??????2002?,???????????John B
    Fenn,???????????????????????????????

14
  • ?Maldi????,???????????????????????????,???????????
    ??????????
  • ????????????,??????????????500010000????,????????
    ??????????,??????????????????????????
  • Maldi?????????????????????Maldi??????????????????,
    ??????????????????????????????????????????????33
    7nm ???????????????,???????????????????????,??????
    ???????????????????,?????1000000???????????????

15
Madil ?Tof????????
  • Maldi????????Tof(????)??????????Maldi-Tof???,?????
    ????????????????????????????,???????,?????????????
    ???????????,????????????????????,?????????,???????
    ?????,???????????Maldi-Tof???????????????????,????
    ????????????????????????,????????????????????????
    ????????????????????????????

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ESI-MS???
  • ????????????????????????,????????????????????,????
    ????????????? ?
  • ???MALDI,??? ?????????,????????????

19
????
20
  • ??, ????????????????, ???????? ???????????????????
    ??, ?????????????, ????(???)???????(collision
    induced dissociation, CID) ?????????????

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????
  • 1.???????????????,????????????? ?
  • 2. ????????

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ICAT??
??????????(isotope-coded affinity
tag,ICAT)??????????????????????,??????????????????
?1999?,Gygi????????????????????????,??????????????
,???????(??????)????,??????????????????,??????????
????????,????????,????????????????????????????????
???????????????????????????????????????????,??????
?????????
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????????????,?????????ICAT????????ICAT????????????
??Cys????,??????,???????,????????,HPLC??,???????IC
AT?????????????
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  • ????????????,?????????ICAT????????ICAT????????????
    ??Cys????,??????,???????,????????,HPLC??,???????IC
    AT???????????????ICAT?????????????????????????????
    ??,????????8???MS/MS??????????????,?????????,?????
    ?????????????????

29
ICAT???
  • ICAT????????,?????(1)???????????????????????????
    (2)????????????????(?SDS????????)???????(3)?????C
    ys?????,?????????????????(4)ICAT?????????????????
    ?????,????????????????

30
?????????????
  • ????????????????( surface-enhanced laser
  • desorptionPionization time of flightmass
    spectrometry , SELDI
  • TOF/MS)??Ciphergen????,?????????????,
  • ????????????????????????????(?
  • ???????????????????)?????(??-???
  • ??-???DNA-???)???,?????,?????????
  • ????TOF/MS????,?????????????????
  • ????,?????????????????,???????
  • ????????(???????)?

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?????
  • ??????????????????????????????????????????????????
    ??????????

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