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Diapositiva 1

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Title: Diapositiva 1 Author: Elisa Last modified by: Jill Filler Created Date: 11/14/2008 7:48:11 AM Document presentation format: A4 Paper (210x297 mm) – PowerPoint PPT presentation

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Title: Diapositiva 1


1
Fabio Arturo Iannotti, Elisabetta Panza, Vincenzo
Barrese, Davide Viggiano, Maria Virginia
Soldovieri, and Maurizio Taglialatela.
Expression, Localization, And Pharmacological
Role Of Kv7 Potassium Channels In Skeletal Muscle
Proliferation, Differentiation And Survival After
Myotoxic Insults. Journal of Pharmacology and
Experimental Therapeutics
A
Myoblasts
Myotubes
a
b
a
60x
B
MW (kDa)
MyHC
220
a-tubulin
55
Supplemental Figure 1. In vitro differentiation
of C2C12 myoblasts. (A) Phase-contrast light
transmission images of murine C2C12 cell
incubated in growth media (GM, myoblasts, a), or
in differentiating media for 72 hours (DM,
myotubes, b). Confocal immunofluorescence images
of the same cells exposed to GM or after
incubation in DM for 72 hours are shown in panels
c and d, respectively. Red Myosin Heavy Chain
(MyHC) protein detected by CY3-conjugated
antibodies green nuclear staining with
chromomycin A3. (B) Western blots of total cell
lysates from myoblasts (GM) and myotubes (DM for
72 hours). The upper panel shows the expression
of the MyHC protein (220 kDa) the lower panel
shows the expression of a-tubulin (55 kDa).
Supplemental Fig. 1
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