Diapositiva 1

1
a
Insulin/Glucose axis-unrelated genes
Insulin/Glucose axis-related genes
gt 7-fold
Gene expression (fold-increase vs. untreated
control)
Supplemental Figure 1
2
b
Supplemental Figure 1
3
a
Adipocytes
Tumor cells
Control
Day 0
DNA FASN
DNA FASN
3T3-L1
MCF-7
CELL GROWTH INHIBITION
ADIPOCYTE MATURATION
FASN down-regulation
FASN up-regulation
3T3-L1
MCF-7
DNA FASN
DNA FASN
Day 9
Metformin
Supplemental Figure 2
4
b
EXERCISE
CALORIE DEPRIVATION
AMP
METFORMIN
ATP
THIAZOLIDINEDIONES
Other AMPK activators
AMPK
LOW-ENERGY STATUS
INACTIVE
TSC2
TSC1
P
P
ACUTE EFFECT
AMPK
ACTIVE
P
P
mTOR
ACACA activity
CHRONIC EFFECTS
4E-BP1
S6K1
Protein synthesis initiation
FASN gene transcription
FASN mRNA
FASN mRNA translation
FASN promoter
Supplemental Figure 2