Development of Microfluidic Glucose Sensors - PowerPoint PPT Presentation

Loading...

PPT – Development of Microfluidic Glucose Sensors PowerPoint presentation | free to download - id: 7af3b2-ZGEzY



Loading


The Adobe Flash plugin is needed to view this content

Get the plugin now

View by Category
About This Presentation
Title:

Development of Microfluidic Glucose Sensors

Description:

Kristen Jevsevar Jason McGill Sean Mercado Rebecca Tarrant Experimental Setup A clamp is used to seal the PDMS to the ceramic electrode Solution containing glucose is ... – PowerPoint PPT presentation

Number of Views:14
Avg rating:3.0/5.0
Slides: 13
Provided by: Sean217
Category:

less

Write a Comment
User Comments (0)
Transcript and Presenter's Notes

Title: Development of Microfluidic Glucose Sensors


1
Development of Microfluidic Glucose Sensors
  • Kristen Jevsevar
  • Jason McGill
  • Sean Mercado
  • Rebecca Tarrant

2
Problem Statement
  • Cells consume different amounts of glucose under
    different environmental situations
  • Problem
  • How to quantify glucose or other metabolite
    consumption/production on a scale of microliters?

3
Performance Criteria
  • Needs to work for at least 24 hours
  • Should recalibrate automatically to account for
    electrochemical drift
  • Must be able to measure glucose concentrations
    between 0mM and 6mM

4
Primary Objective
  • Design a glucose electrode interface that will
    measure micro-scale concentrations while
    maintaining affordability

5
Solution Description
  • Pine Instruments makes a ceramic platinum screen
    printed electrode that we will be interfacing to
    a microfluidic system

6
Goals
  • Interface a microfluidic channel with our ceramic
    electrode
  • Create a pumping system which can self-calibrate
  • Make it more affordable

7
Factors
  • Smaller volumes yield more accurate results
  • PDMS and ceramic electrodes are inexpensive

8
Performance Metrics
  • Calibration curves
  • Linear correlation
  • Measurable range of concentrations
  • Flow capacity
  • Control tests with known concentrations

9
Experimental Setup
  • A clamp is used to seal the PDMS to the ceramic
    electrode
  • Solution containing glucose is run through the
    channel and measured

10
Previous Work
  • A clamp was designed for proper sealing
  • Rough PDMS channels were made to fit the ceramic
    electrode
  • Calibration curves of the electrode simply in a
    beaker containing differing amounts of glucose
    have been made

11
Current Work
  • Designing a more reproducible channel using a CNC
    end mill
  • Setting up a calibration experiment for stop/flow
    and continuous pumping

12
Future Work
  • Testing the sealing characteristics of the new
    design
  • Testing the system while using cell media
  • Designing a pumping system for the PDMS/ceramic
    electrode component
About PowerShow.com