3%20D%20Structure%20of%20Immunoglobulins - PowerPoint PPT Presentation

View by Category
About This Presentation
Title:

3%20D%20Structure%20of%20Immunoglobulins

Description:

... Space filling model Mulptiple Myeloma Disease Plasmocytoma Cancer of antibody producing cell Symptoms recurrent infections, anaemia, fractures, pain, ... – PowerPoint PPT presentation

Number of Views:217
Avg rating:3.0/5.0
Slides: 46
Provided by: Hugh136
Category:

less

Write a Comment
User Comments (0)
Transcript and Presenter's Notes

Title: 3%20D%20Structure%20of%20Immunoglobulins


1
3 D Structure of Immunoglobulins
  • Hugh B. Fackrell
  • FilenameKuby05b7.ppt

2
  • Assigned Reading
  • Content Outline
  • Performance Objectives
  • Key terms
  • Key Concepts
  • Short Answer Questions

3
ASSIGNED READING
  • Chapter pp

4
Antibody structure
  • Special antibodies to study structure
  • Myeloma proteins
  • Bence Jones Proteins
  • Monoclonal Antibodies
  • 3D structure of antibodies
  • Binding site
  • Variability in structure

5
Angels Devils
6
EM of IgA
7
Antibody flexibility
8
Antibody Space filling model
9
Mulptiple Myeloma
  • Disease
  • Plasmocytoma
  • Cancer of antibody producing cell
  • Symptoms
  • recurrent infections, anaemia, fractures, pain,
    bone lesions
  • Detection
  • Electrophoresis, X-rays, blood smear

10
IgG Crystal
11
Myeloma Induction
12
Bence Jones Protein
  • Homogenous light chains
  • In urine of myeloma patients
  • Easily purified
  • Insoluble at 60 -70 oC
  • Soluble at 80 oC

13
Monoclonal Antibodies
  • Add information here

14
Antibody model
15
Problems with flexibility
  • Difficult to determine entire structure
  • Hinge flexibility
  • Fragment
  • Study Fragment
  • Assemble composite

16
Hinge Prolines
17
IgG Enzyme hydrolysis
18
Bence Jones Light chains
  • Simplest fragments to isolate
  • Easily crystallized
  • Mimics Fab

19
Bence Jones Monomer
20
Bence Jones Dimer
21
Immunoglobulin fold
  • Signature structure common to all immunoglobulins
  • 2 beta sheets
  • held by disulfide bond
  • Variations in detailed structure set specificity

22
Ig Fold Variable domains
23
Ig fold Constant domains
24
Antigen Binding Site
25
Structure of IgG
26
Ig Fold hypervariable regions
27
Vitamin K bound to IgG
28
Antigen binding to Antibody
29
Antibody Strucutre Internal motion
30
Antibody Structure Internal movement
31
Antigen in Antibody
32
Hypervariable Regions
  • Discovery
  • KW plots
  • Light chains
  • Heavy chains
  • CDR vs HVR

33
Kabat Wu Plots
  • Why
  • demonstrate details of variability of protein
    sequence of similar proteins
  • Results
  • Variability 1.0 gtgt no variation
  • Variability 400 gtgt 20 different AA

Number different amino acids
Variability
Frequency of most common AA
34
HV light chains
35
KW plot heavy chains
36
HVIgG
37
KW Heavy chains CDR
38
KW Light chains CDR
39
Ig Superfamily
40
Isotypes Allotypes Idiotypes
41
Allosera Production
42
Isotypes, Allotype Idiotype
43
The End
44
Performance Objectives
  • Key terms, concepts
  • short answers

45
SHORT ANSWER QUESTIONS
About PowerShow.com