Title: Supplemental Figure S1. Effects of incubation conditions on the accumulation of vacuole-targeted GFP in vacuolar lumens. A, A Fresh leaf excised from vacuole-targeted GFP expressing plants (Tamura et al., 2003) was observed immediately by LSCM. B, C and
1Supplemental Figure S1. Effects of incubation
conditions on the accumulation of
vacuole-targeted GFP in vacuolar lumens. A, A
Fresh leaf excised from vacuole-targeted GFP
expressing plants (Tamura et al., 2003) was
observed immediately by LSCM. B, C and D, Excised
leaves from vacuole-targeted GFP expressing
plants at 30 days after sowing were incubated at
23C for 20 h in 10 mM MES-NaOH with 1 µM
concanamycin A and 100 µM E-64d in darkness (B),
with MS medium containing sucrose in darkness (C)
or in the light (D). GFP appears green and
chlorophyll fluorescence appears red. In B, C and
D, merged images are shown. Bars50 µm.