AFCG Biosafety Guidelines for Analysis and Sorting of Unfixed Cells.

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AFCG Biosafety Guidelines for Analysis and
Sorting of Unfixed Cells.
AFCG website
ScopeThis document RECOMMENDS procedures to be
undertaken during the analysis and sorting of
potentially biohazardous material on Flow
Cytometers.
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AFCG Guidelines 2006 - Introduction Page 2 of
guidelines AFCG website FOREWORD These
recommendations are presented with a view to
being a minimum standard and should not be seen
to restrict the ability of any individual. This
document will be reviewed regularly to ensure
that these recommendations embrace current
accepted laboratory practices. These guidelines
are written in broad terms as a gesture to
indicate and not to dictate.
HOWEVER
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EMPLOYER RESPONSIBILITIES

• SO FAR AS IS REASONABLY PRACTICABLE
• Provide and maintain a safe
• working environment
• plant or systems of work
• workplace in a condition that is safe and without
  risk
• system for the use, handling, storage or
  transport of plant or substances

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Biohazardous material All unfixed material of
human origin. All unfixed material of primate
origin. All human peripheral blood leukocytes,
bone marrow, splenocytes, thymocytes, sperm
cells, Cells from primary and immortalized
cultures from humans, primates, and transgenic
animals. All genetically modified organisms
(GMO) Micro-organisms. Special care to be
taken for brain tissue (prions) and lung
lavage (tuberculosis).
WHY ?
TO PROTECT THE OPERATOR , THE USERS AND THE
ENVIROMENT.
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GENERAL CONSIDERATIONS

1. Policies

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GENERAL CONSIDERATIONS
2) BEFORE A NEW USER STARTS Flow Cytometry
tutorials are mandatory, they cover theory
practice and safety.
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GENERAL CONSIDERATIONS
3) NOTIFICATION
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Implementation
Handling of biohazardous material gtgt IN
TRANSIT AND PREPARATION STANDARD PC2 page
2 AFCG Guidelines
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Implementation
REFERENCES page 14 of guidelines
www.ogtr.gov.au/ Biosafety Guidelines for
Sorting of Unfixed Cells, Cytometry, 2899-117
(1997). Cytometry A. 2007 Jun 71(6)414-37.
International Society for Analytical Cytology
biosafety standard for sorting of unfixed
cells. Schmid I, Lambert C, Ambrozak D, Marti
GE, Moss DM, Perfetto SPInternational Society of
Analytical Cytology. Biosafety Considerations
for Flow Cytometric Analysis of HIV infected
Samples, Cytometry Comm., 38195-200 (1999).
Novel Rapid Method for Visualization of Extent
and Location of Aerosol Contamination During
High-Speed Sorting of Potentially Biohazardous
Samples, Cytometry, 42217-22 (2001). Measuring
Containment of Viable et Infectious Cell Sorting
in High-Velocity Cell Sorters. Perfetto et al.,
Cytometry Part A 52A122-130(2003) Viable
Infectious Cell Sorting in a BSL-3 Facility, Flow
Cytometry Protocols, vol. 263, Humana Press,
2004 Biohazard Sorting, Methods in Cell
Biology, Cytometry, 4th ed., vol.75,2004Oberyszyn
A.S. Robertson F.M. .Novel rapid method for
visualization of extent and location of aerosol
Contamination during high speed sorting of
potentially biohazardous samples. Cytometry
2001 43 217-222 Current Protocols in
Cytometry Jan 2007 UNIT 3.6 Standard Safety
Practices for Sorting of Unfixed Cells. Ingrid
Schmid1, Claude Lambert2, David Ambrozak3, and
Stephen P. Perfetto3 .Current Protocols in
Cytometry Feb 2002 UNIT 3.5 Method for
Visualizing Aerosol Contamination in Flow
Sorters. Andrew S. Oberyszyn1 .WHO
Biosafety Manual.http//www.who.int/csr/resources/
publications/biosafety/who
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Implementation / Testing
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Implementation / Testing

• IN HOUSE PROCEDURES

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SURVEILLANCE

• IN HOUSE PROCEDURESgt work practices

• Passaged cells , how many times
• New work , ask what type
• Unsure , ask IBC

Ask the question
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MANAGEMENT
OHS requirements IBC Internal Biosafety
Committee AFCG guidelines Line manager
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Decontamination Procedures