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Nucleic acids

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Sarote Boonseng Deoxyribonucleic acid (DNA) Ribonucleic acid (RNA) ... – PowerPoint PPT presentation

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Title: Nucleic acids


1
Nucleic acids
  • Sarote Boonseng

2
Introduction
  • ????????????????????????????
  • Deoxyribonucleic acid (DNA)
  • Ribonucleic acid (RNA)
  • ?????????????????????????

???????? ????????? ???????????? ???????????
????????????????? DNA ??????? cytoplasm
3
???????????? (DNA RNA)
4
?????????
  • ??????????????????
  • ??????
  • ??????

monophosphate
diphosphate
triphosphate
5
Nucleoside
Nucleoside sugar base
N-glycosyl linkage
6
Nucleotide
Nucleotide phosphate sugar base
5
5
phosphoester
7
????????????????????
Nucleotide Abbreviation
Adenosine 5-monophosphate Adenosine 5-diphosphate Adenosine 5-triphosphate Deoxyadenosine 5-monophosphate Guanosine 5-monophosphate Cytidine 5-monophosphate Uridine 5-monophosphate Deoxythymidine 5-monophosphate AMP ADP ATP dAMP GMP CMP UMP dTMP
8
DNA RNA
Deoxyribonucleic acid (DNA) A G T and
C Ribonucleic acid (RNA) A G U and C
9
Watson-Crick base-pairing
10
DNA
  • ?????????????????????? ??????? ???????????????????
    ???
  • ????????????????????????? DNA ???? Antiparallel
  • ?? DNA ?????????????? ?????????
  • DNA ????????????????????? RNA
  • DNA ?????????????????????? nucleosome (DNA ??????
    Histones) ?????????????? chromosome

11
RNA
  • ????????????????????????? ??????????????????????
    ????????????????????????? Hair-pin structure
  • ?? RNA ?? cytoplasm ???
    Nucleus
  • ???????? 3 ????
  • mRNA ???????????????????????
  • rRNA ?????????????????????????
  • tRNA ???????????????????????????????



????????? ???????????
??????? RNA ?????????????????
12
Properties of nucleic acid
  • Denatured DNA
  • Break down H-bonds by heat or alkaline
  • ?????????????? DNA ?????????????????????

Supercoiled form Linear form Circular form
D Supercoiled gt Linear gt Circular
????? http//www.sbs.utexas.edu/395J/395J_2009/Re
adings/Lecture202/Supercoiling.pdf
13
?????????????????????
H/heat
DNA/RNA
Base
Sugar
PO4
Deoxyribose
Ribose
Diphenylamine
Orcinol
Purple color
Green
13
14
????????????????
DNA ?????????????????????? RNA ???????????????????
????? ????? C2 ??? RNA ???? OH ????
rearrangement ??? phosphate
14
15
The End
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