Microscopy fits needs of researcher, type of specimen being viewed

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Microscopy fits needs of researcher, type of
specimen being viewed

• Brightfield
• Darkfield
• DIC/Nomarski
• Ultrastructural
• Dimensional microscopy (SEM and Confocal)
• Simple magnification

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Resolution Depends Upon Magnification, Type of
Scope, Specimen Fixation, Thickness, Staining

• Staining types General
• Acid stains (eg., eosin, orange G, aniline blue),
  
• Basic stains (Methyl green, methylene blue)
• Mordants (Hematoxylin, a generally basic dye, is
  a mixture of hematoxylin and mordant (link b/w
  dye and tissue) generally used in connection with
  acidic dyes to provide contrast and staining of
  cellular components
• Specific tissue component stains (eg., PAS for
  carbohydrates)

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Resolution, contd

• Staining Types - Specific
• Immunohistochemistry
• Enzyme Histochemistry
• Autoradiography

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Immunohistochemistry
Fluorescent or enzymatic label
Primary antibody
Secondary Antibody
Microscope Slide
Protein in tissue
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Enzyme Histochemistry
Substrate
Enzyme in cells of tissue
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Autoradiography

• Incorporation of label into DNA
• Injection of labelled tracer into animal or cell
• Removal of tissue, fixation, sectioning
• Exposure of section to photographic emulsion
• Emission of radioactive tracer reacts with
  emulsion
• Emulsion is developed, silver grains denote label
  location
• Counterstain and view
• Areas of label indicative of cellular process
  (eg., making DNA, protein) being examined

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Guess the Technique
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- control
control)
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control
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