Production of monoclonal antibodies - PowerPoint PPT Presentation

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Production of monoclonal antibodies

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Immunisation . Mice are immunised with Ag Spleen cell,lymphocytes suspension is obtained . Antigen must be greater than 3000-5000 Da or linked to a ... – PowerPoint PPT presentation

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Title: Production of monoclonal antibodies


1
Production of monoclonal antibodies.
  • ABHILASHA TIWARI
  • ANKITA DWIVEDI
  • JASMINE KAUR
  • ARIJIT BISWAS

2
  • Monoclonal anitbodies are homogeneous population
    of antibody molecules,derived from a single
    antibody producing cell,in which all antibodies
    are identical and of the same precise specificity
    for a given epitope.

3
Steps in production of monoclonal antibodies
4
Immunisation.
5
Fusion.
6
Culture in HAT.
7
(No Transcript)
8
Screening.
  • ELISA
  • RIA
  • SOLID PHASE IMMUNOASSAY..
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