Production of monoclonal antibodies - PowerPoint PPT Presentation
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Production of monoclonal antibodies
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Immunisation . Mice are immunised with Ag Spleen cell,lymphocytes suspension is obtained . Antigen must be greater than 3000-5000 Da or linked to a ... – PowerPoint PPT presentation
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Title: Production of monoclonal antibodies
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Production of monoclonal antibodies.
- ABHILASHA TIWARI
- ANKITA DWIVEDI
- JASMINE KAUR
- ARIJIT BISWAS
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- Monoclonal anitbodies are homogeneous population
of antibody molecules,derived from a single
antibody producing cell,in which all antibodies
are identical and of the same precise specificity
for a given epitope.
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Steps in production of monoclonal antibodies
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Immunisation.
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Fusion.
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Culture in HAT.
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Screening.
- ELISA
- RIA
- SOLID PHASE IMMUNOASSAY..
