PGBA4 / CGBA2 Payload on ISS.9A BioServe Space Technologies A Life Sciences - PowerPoint PPT Presentation

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PGBA4 / CGBA2 Payload on ISS.9A BioServe Space Technologies A Life Sciences

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PGBA4 / CGBA2 Payload on ISS.9A BioServe Space Technologies A Life Sciences NASA Commercial Space Center CGBA PGBA Alex Hoehn Assoc. Director Engineering – PowerPoint PPT presentation

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Title: PGBA4 / CGBA2 Payload on ISS.9A BioServe Space Technologies A Life Sciences


1
PGBA4 / CGBA2 Payload on ISS.9ABioServe Space
TechnologiesA Life Sciences NASA Commercial
Space Center
CGBA
PGBA
  • Alex Hoehn Assoc. Director Engineering
  • BioServe Space Technologies, 429, UCB, Boulder,
    CO 80309-0429, Tel. (303) 492-1005, Fax (303)
    492-8883

2
PGBA-4 STS-112 / ISS.9A
3
PGBA-4 STS-112 / ISS.9A
4
PGBA-4 STS-112 / ISS.9A
  • Payload Overview PGBA-4 / STS-112 ISS.9A -gt
    ISS.11A Inc.5
  • Science Summary.
  • Reflight (4th) of PGBA, first ISS flight.
  • Modifications since STS-94.
  • Split coolant system / dual pump.
  • Modified nutrient delivery for timed germination.
  • Plant Access on Orbit
  • Open Payload on Orbit
  • Reverification of plant chamber seal.

5
Science Overview
  • Investigate time course of genetic changes due to
    microgravity exposure
  • 30 Arabidopsis thaliana plants
  • Biologically simple (mustard-type plant).
  • Fast growth rate matures in 4-6 weeks.
  • Entire genome sequenced.
  • Two crops during Inc.5, automated, time-
    staggered germination, some germinated
    pre-launch
  • 1st crop sown pre-flight, multiple automated
    germinations, single harvest, fixed on-orbit.
  • 2nd crop sown in-flight, automated germination,
    fixed on ground post-flight.

Mature plant, 4-6 weeks.

Plant as harvested from PGBA, 3 weeks.
6
PGBA Payload Overview
PGBA-03 STS-94 in Proto-EXPRESS / Spacelab.
PGBA-04 Mufflers for ISS.
STS-77 10 day mission STS-83 4 day aborted
MSL-1 STS-94 16 day MSL-1
No PGBA anomalies,but - transfer issues / lesson
learned
7
Transfer Operations PGBA to / from ISS
PGBA Transfer Middeck to Spacelab, STS-94.
8
Operations Open PGBA Faceplate
Plant Chamber lid still closed Inspect for free
water.
  • 4 locker latches open.
  • Faceplate rotated around bottom edge (hinged).

9
Remove Plant Chamber Lid Screws
10 captive fasteners (1-2 turns). Pull lid
uniformly (parallel) from chamber.
10
Harvesting Plant
Reach in, rotate each insert 2-3 turns ccw, then
remove insert with plant. Repeat for all 30
plants, fix and store in CGBA. Insert new seed
plug, rotate cw until tight.
Harvesting / Insertion of new seed plug (30x)
into PGBA.
11
ISS Operations Harvesting
Fixative Tube
Plunger rod connect after plant insertion
End cap remove to insert plant
  • Remove fixative tube from CGBA, harvest plant,
    place plant in tube, attach plunger rod, inject
    fixative - slowly, store in CGBA.
  • Repeat for entire PGBA crop 30x.

12
Operations Sowing
Removable Plug
  • Place new dry seed plugs into PGBA root tray, one
    at a time (30x).
  • Close chamber lid, hand-tighten 10 captive
    fasteners 2-3 times to ensure good seal.
  • Close PGBA faceplate, aligning pins and locking
    latches (4x).
  • Reconnect cables, power up PGBA.
  • Replace mufflers.
  • Perform PGBA Status Check.

Plastic Covered
Steel Wire
Small Plastic
Centrifuge
Tube
Plastic
Felt
Line
13
Re-Attach and Re-seal Plant Chamber Lid
Tighten screws 2-3 times around
Attach chamber lid and push down. Tighten 10
captive fasteners (1-2 turns) going around
perimeter until finger-tight. Finger-tighten 1-2
more times to ensure proper seal. (Optional
battery-powered screw driver with Phillips head
bit.)
5
2-3 times
4
1
2
3
14
PGBA Plant Chamber
15
Atmosphere Treatment System
16
Atmosphere Treatment System
  • Atmosphere Treatment Cartridge
  • Barium Hydroxide
  • Activated Charcoal
  • 3 levels of containment
  • Stainless steel frit
  • Glass mesh
  • Stainless steel screen

17
Humidity Control / Condensate Recovery
18
PGBA-4 Pictures
19
PGBA-4 Pictures
20
PGBA-4 Pictures
21
PGBA-4 Pictures
22
PGBA-4 Pictures
23
PGBA-4 Pictures
24
PGBA-4 Pictures
25
PGBA-4 Pictures
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