Ljubljana, Slovenia

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Ljubljana, Slovenia
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Components of the antiviral defense device
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Therapeutic targets of HIV life cycle
www.mja.com.au
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Considered approaches
Use of viral LTR promoter Leaky transcription
Direct activation of antiviral response (e.g. TLR3 ?interferon ß) Problems with active homodimers
Detection of viral attachment Selected approach
Use of viral protease activity Selected approach
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Localization-based switch of T7 RNA polymerase
activation
Active state T7 RNA polymerase is released from
the membrane and directed to the nucleus (NLS)
transcription of antiviral effectors
Inactive state T7 RNA polymerase is anchored to
the membrane and is separated from DNA
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HIV causes the release of GFP reporter from the
membrane
3D reconstruction of reporter GFP localization
within the cell
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HIV protease causes translocation of mCherry
reporter into the nucleus
Membrane localization
Nucleus localization
control
HIV protease
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HIV protease - based cell activation
Control
HIV protease
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