How was DNA determined to be the genetic material?

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How was DNA determined to be the genetic material?

• A connection between data and conclusions!
• A picture is worth 1000 words!!!

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Your Assignment

• Identify the purpose of the given experiment.
• State your scientists hypothesis.
• Explain the methods (include new vocab!).
• Explain the data they got.
• Connect the experiment to the conclusion about DNA

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Griffith and Avery
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Hershey and Chase
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Photo 51 and Chargaffs Rules (Watson and Cricks
Model)
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Messelson and Stahl
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DNA Replication and Enzymes

• Using a video, review the process of DNA
  replication
• Identify the role of the following enzymes
• Helicase, DNA polymerase (more than one role),
  nuclease, single-stranded binding protein,
  ligase, primase
• Classify enzymes as leading strand replication,
  lagging strand replication, proofreading (may
  have more than one function
• Clarify the direction the template strand is read
  and the place on the new strand where a
  nucleotide is added. Identify the role of the
  additional phosphate groups on the nucleotide.

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Summary of Replication
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Beyond the double helix DNAs organization
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Histones

• Material Protein
• Function Organize DNA
• Big Idea 2 (Regulation)What does acetylation do?

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DNA Methylation

• Adding a CH3 to the DNA backbone can shut off the
  gene from working.
• Gene sequence of DNA that codes for a
  particular protein

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Big Idea 2 3 The function of DNA can be
controlled at the DNA structural level!

• This is epigenetics!
• Our epigenetics friends will delve further into
  this )

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Chromosome vs. Chromatin

• Almost a continuum
• When does DNA condense into chromosomes?
• Why does the cell expend energy to organize the
  DNA into chromosomes?