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Synthetic Biology Review

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Title: Critical Review Author: Tom Adie Last modified by: Tom Adie Created Date: 7/23/2008 10:18:23 AM Document presentation format: On-screen Show (4:3) – PowerPoint PPT presentation

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Title: Synthetic Biology Review


1
Synthetic Biology Review
A Synthetic Oscillatory Network of
Transcriptional Regulators The Repressilator
Elowitz, M.B. Leibler, S.
Nature, vol. 403, 20th January 2000
2
The Basics
3
The Good Results...
4
...and the Other Results
  • ? Limitations (c)
  • Accumulation of GFP as a result of limited
    elimination
  • Green oscillation with a reduced period
  • Blue several periods skipped entirely
  • ? Controls (f)
  • Cells containing only the reporter plasmid

Stochastic effects
5
Conclusions Extensions
  • ? iGEM Solution
  • Novel approach based on Lotka-Volterra model of
    predator-prey interaction
  • Constitutively repressed predator cell is
    anti-repressed by product of prey cell
  • Prey cell is repressed by product of predator
    cell
  • Thus a stable oscillation cycle is built up

Cell A
A
Lux R
Lux I
Plux
A
Cell B
A
A
LuxR
AiiA
LuxR
Lux R
aiiA
Plux
6
The Network Model
Deterministic Continuous / Stochastic Discrete
Continuous model
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