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Title:

Introducing automated ELISA - ChemWellTM

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Title: PowerPoint Presentation Author: Sergio Lopez Created Date: 8/15/2001 3:07:29 AM Document presentation format: On-screen Show Company: Home Other titles – PowerPoint PPT presentation

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Title: Introducing automated ELISA - ChemWellTM


1
Introducing automated ELISA - ChemWellTM
  • Geoffrey Anderson
  • Unitech Scientific LLC
  • Lakewood, CA
  • Visit us
  • www.unitechscientific.com

2
ChemWellTM Wine Autoanalyzer
Manufactured by Awareness Technology Unitech
Scientific - Exclusive Distributor
3
First analyzer in the world to combine
biochemistry and microplate EIA into one
instrument.
ChemWell uses microwells for all reactions,
chemistry and EIA.
4
A ChemWell Tour
Optical system
2 syringe pumps
Wash head
Windows 95, 98 or XP
Wash and rinse bottles
reactions
Reagents
Samples
DI water
5
ChemWell
Designed for labs doing 100 - 1000 tests per
day or 400 EIA per 8 hours

Recommended workload
6
  • ChemWell Features Benefits
  • Easy to Use
  • Continuous Sample Loading
  • Stat sample priority
  • Low Operating Cost
  • Intuitive, Open Software
  • Proven, Reliable Technology

7
Calibration Options
  • Cut-off (Semi Qualitative)
  • Multi-point Polynomial
    fit
  • Cubic Spline fit . . . and more
  • Store Calibration Curves
  • Wine Controls Chart Levy Jennings

8
ChemWell runs ELISA as well as Enzymatic reagents
  • Unitech offers Histamine ELISA kits
  • Botrytis and Aspergillus ELISA kits
  • are in development
  • So lets take a quick review of the steps and
    principals of ELISA reagents.

9

Immuno Complex an Antigen with its matching
Antibody
Ag stands for Antigen
Ag
Ab stands for Antibody
Ab
An antigen and its antibody fit together like 3
dimensional puzzle pieces.
10
Diagram of a simple ELISA with a positive sample
(sample has Ag in it)
  • Mix the sample and diluent together in a
    microwell that is pre-coated with specific Ab
  • Incubate for x minutes until the Ag in the sample
    binds to the Ab on the well walls.

Start with an Add the sample
Incubate until Ab-coated well with some
Ag in it complexed
Ag
Ab
Ab
Ab-Ag
Ag
Ab-Ag
Ab
Ab
Ab
Ab
Ab
Ab
11
Conjugate is a specific Ab bound to an Enzyme.
Wash out the Add a
Incubate until remaining sample
conjugate complexed
Ab-E
Ab-Ag-Ab-E
Ab-Ag
Ab-Ag
Ab-E
Ab
Ab
Ab
Ab-Ag-Ab-E
Ab-Ag
Ab-Ag
Ab-E
Ab-E
The conjugate has a second antibody to the same
antigen, allowing you to make an antigen sandwich
(with antibody bread). It also leaves an enzyme
hanging off.
12
Washing . . . removes the unbound conjugate,
without removing bound antibodies.
Wash out the Add a
Substrate Enzyme remaining conjugate
substrate produces color
S
Ab-Ag-Ab-E-S
Ab-Ag-Ab-E
Ab-Ag-Ab-E
Ab
Ab
Ab
Ab-Ag-Ab-E-S
Ab-Ag-Ab-E
Ab-Ag-Ab-E
S
S
S
The more Enzyme available, the more color will
develop. Many ELISAs use a stopping solution to
end the color generating step before reading.
13
Reliable Results Enzymatic and ELISA
  • Precise pipetting
  • Washes probe inside and out
  • Liquid sensing
  • Temperature controlled

14
Easy to Maintain
  • Proven Reliability, over 500 sold
  • Self Diagnosis
  • Phone/email support by Unitech
  • Modular Design

15
Economical to Use
  • Dramatically reduces LABOR
  • Laboratory
  • Administrative

16
Warranty
  • 1-year parts labor Warranty
  • Extended Warranty available
  • 6-Month Preventative Maintenance
  • Free replacement of warranteed parts
  • Other repairs if required
  • Loaner instrument within 72 hours

17
Easy to own
  • Price start at 27,500
  • Reagent rack cooling option
  • Add 2000

18
ChemWell Distributed by Unitech Scientific
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