Rhizobium, has the ability to fix atmospheric nitrogen in symbiotic association with host legumes. - PowerPoint PPT Presentation

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Rhizobium, has the ability to fix atmospheric nitrogen in symbiotic association with host legumes.

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Rhizobium as component of INM INTRODUCTION Rhizobium, has the ability to fix atmospheric nitrogen in symbiotic association with host legumes. – PowerPoint PPT presentation

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Title: Rhizobium, has the ability to fix atmospheric nitrogen in symbiotic association with host legumes.


1
Rhizobium as component of INM
INTRODUCTION
Rhizobium, has the ability to fix atmospheric
nitrogen in symbiotic association with host
legumes.
Rhizobia invade the roots of legume plants
through root hairs, form effective pink colored
nodules in the tap or lateral roots and lives
symbiotically inside the nodules to fix nitrogen
to plant accessible forms
It was estimated that a total of 175 million
metric tones of N is fixed per year globally
through biological nitrogen fixation
involving Legume Rhizobium symbiosis.
2
Rhizobium as component of INM
Rhizobium, a root nodule bacterium, has the
ability to fix atmospheric nitrogen in
symbiotic association with host legumes.It
was estimated that a total of 175 million
metric tones of N is fixed per year globally
through biological nitrogen fixation
involving Legume Rhizobium symbiosis.
3
Rhizobium as component of INM
HOW RHIZOBIA INFECT LEGUME ??
  • Curling of root hair
  • Invagination point
  • Infection thread develop
  • Bacteria released
  • Nodular tissue develop

4
ISOLATION AND MAINTENANCE OF RHIZOBIUM
Selection of legume crops
Selection of legume crops
Selection of legume crops
Selection of legume crops
Selection of legume crops
Selection of legume crops
Collection of nodules
Collection of nodules
Collection of nodules
Collection of nodules
Collection of nodules
Collection of nodules
Rhizobium isolation in media
Preservation in slant
Rhizobium isolation in media
Preservation in slant
Rhizobium isolation in media
Preservation in slant
Preservation in slant
Rhizobium isolation in media
Preservation in slant
Nodulation tests
Purified rhizobial cells
Nodulation tests
Purified rhizobial cells
Purified rhizobial cells
5
Rhizobium as component of INM
CHARACTERISTICS OF RHIZOBIUM
Rhizobia invade legume roots through
root hairs Form effective pink colored nodules
in the roots Lives symbiotically inside the
nodules and fix nitrogen Converts atmospheric
nitrogen to plant accessible forms Most
rhizobia are host specific in nature
6
Rhizobium as component of INM
RHIZOBIUM CONTRIBUTES
50 200 KG NITROGEN PER HECTARE PER CROP
7
Rhizobium Inoculation and Crop Response
Inoculation Method
Rhizobium culture
Nitrogen Fixation
Crop Response
8
Rhizobium as component of INM
Contribution of Rhizobium in biological N fixation
9
Rhizobium as component of INM
LET US SUM UP
Rhizobium fix atmospheric nitrogen in symbiotic
association with host legumes. It was
estimated that a total of 175 million metric
tonnes of N is fixed per year through
legume-Rhizobium symbiosis. Rhizobium lives
symbiotically inside the nodules and fix nitrogen
which is converted to plant accessible forms.

Contd..
10
Rhizobium as component of INM
LET US SUM UP
Rhizobia invade the roots of legume plants
through root hairs and form effective pink
colored nodules in the tap or lateral roots.
Most Rhizobia are host specific and nodulate
specific legumes.
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