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Experiment 1 medical microbiology experiment

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Title: Experiment 1 medical microbiology experiment


1
Experiment 1 medical microbiology experiment

2
  • ??????????
  • ???????,??????????
  • ??(??),???????????

3
?????
  • 1.??????????(?????),??????????,??????????????,????
    ???
  • 2.?????????????????????
  • 3.???????????????,??????,????????????
  • ??????
  • 4.?????????,??????????,???????,??????
  • ??????,?????????????????,?????
  • 5.???????????????????????????????????
  • ???????????????????,??????????
  • 6.????,????,???????????
  • 7.???????????????????????????????????
  • ?????????,?????
  • 8.??????????????????????????,???????,
  • ???????????,?????,???????????,??2???75?????????
    ??,???3???5???????,????1????,???????.?????????10
    20???,?????????????
  • 9.????,??????????(???????????)?
  • 10.????,????????????????????????
  • ????????,????? ????,?????????????????????????????
    ????????

4
Microbic Morphology
Medical microbiology experiment (1)
5
  • Content
  • 1. Microbic Morphology
  • ?Bacteria
  • ?Virus
  • ?Fungus
  • 2. Use oil-immersion lens of microscope
  • 3.Bacterial gram stain

6
  • 1. Microbic Morphology
  • ?Bacteria
  • Demonstration
  • ground form
  • coccus(Staphylococcus aureus)
  • bacillus(Escherichia coli)
  • spirobacteria
  • vibrio(Vibrio cholerae)
  • special structure
  • flagellum(Salmonella typhi)
  • capsule(Streptococcus pneumoniae)
  • spore(Clostridium tetani)
  • pilus

7
  • Coccus

8
  • Staphylococcus Streptococcus Diplococcus

9
  • Bacillus

Bacillus anthracis
Escherichia coli
Brucella
10
Corynebacterium
Bifidobacterium
Mycobacterium
Clostridium
11
  • Spirobacteria

Vibrio Spirillum
Helicobacterium
12
  • Special structure
  • Flagellum

13
  • Capsule
  • Spore

14
  • ? Virus

poxvirus poliovirus
rhinovirus
15
tobacco mosaic virus
phage
rabies virus
16
  • ? Fungus

Unicellular fungusyeast,yeast like
fungus,pseudohypha multicellular
fungusfilamentous fungus,mold,hypha and spore?
S. albicans
Aspergillus
17
  • 2. Use oil-immersion lens of microscope

18
  • 3.Bacterial gram stain
  • (Staphylococcus aureus , Escherichia coli)
  • Gram Stain - developed Christian Gram in 1884
  • The gram stain is the most widely used staining
    procedure in bacteriology. It is called a
    differential stain since it differentiates
    between gram-positive and gram-negative bacteria.
    Bacteria which stain purple with the gram
    staining procedure are termed gram-positive
    those which stain pink are said to be
    gram-negative.
  • Gram-positive and gram-negative bacteria stain
    differently because of fundamental differences in
    the structure of their cell walls.

19
  • Principle

Structure of a Gram-Negative Cell Wall
Structure of a Gram-Positive Cell Wall
20
  • Aseptic technique

Aseptic area 10-20cm
??
??
??
21
procedure
  • Smear preparation
  • Smear
  • Dry Fix

aseptic technique
22
  • Stain procedure
  • Initial stain Mordanting
    Decolorize After stain
  • (crystal violet 1) ( iodine solution 1)
    (95 alcohol 30) (diluted carbol


  • fuchsin 30)
  • Rinse Blot Microscopic
    examination

rinse
rinse
rinse
23
Notice
  • 1. Aseptic technique
  • 2. Not too much NS and bacteria
  • 3. Time of decolorization

24
  • Material
  • 1.????????????????1?/??(4?)
  • 2.??1?/??
  • 3.??1?/?

25
  • ?Content of report
  • 1.Bacterial gram stain (Principle, Procedure,
    result)
  • 2.Bacterial morphology (result examined under
    microscope)

26
name magnification result
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