SURVEY OF BIOCHEMISTRY Protein-NA Interactions DNA Replication, Damage, and Repair - PowerPoint PPT Presentation

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SURVEY OF BIOCHEMISTRY Protein-NA Interactions DNA Replication, Damage, and Repair

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SURVEY OF BIOCHEMISTRY Protein-NA Interactions DNA Replication, Damage, and Repair * * What to Know for Exam 1 Structures: NA, AA, Classifications Base pairs ... – PowerPoint PPT presentation

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Title: SURVEY OF BIOCHEMISTRY Protein-NA Interactions DNA Replication, Damage, and Repair


1
SURVEY OF BIOCHEMISTRY Protein-NA Interactions
DNA Replication, Damage, and Repair
2
What to Know for Exam 1
  • Structures
  • NA, AA, Classifications
  • Base pairs, numbering system
  • Polypeptide backbone
  • Dihedral angles
  • Characterization
  • Beers Law
  • Protein Purification and SDS-PAGE
  • pI
  • Processes General Concepts

3
Study Tips
  • Read text
  • Review Lecture Notes
  • Practice problems
  • Read text again

4
PRS
  • Which of the following reagents would be suitable
    for reducing a disufide bond?
  • Methionine
  • 2-Mercaptoethanol
  • Sodium Dodecyl Sulfate
  • Cysteine

5
PRS Question
  • The dihedral angle psi is defined by which of the
    following atoms?
  • N-Calpha-Ccarbonyl-N
  • Calpha-Ccarbonyl-N-Calpha
  • Ccarbonyl-N-Calpha-Ccarbonyl
  • N-Calpha-CcarbonylO

6
PRS Question
  • How many H-bond acceptors in adenine participate
    in Watson-Crick H-bonds with thymine?
  • None
  • One
  • Two
  • Three

7
PRS Question
  • How many H-bond acceptors in adenine participate
    in Watson-Crick H-bonds with thymine?
  • None
  • One
  • Two
  • Three

8
Protein-DNA Interactions
  • Restriction Enzymes
  • Repressor Proteins
  • Transcription Factors
  • Zinc Fingers
  • Leu Zippers
  • Eukaryotic Chromosomes

Specific recognition
Nonspecific recognition
9
Restriction Enzymes
  • H bonds stabilize complex
  • DNA binds in cleft formed by 2 subunits

EcoRI
TCGCGAATTCGCG
10
Helix-Turn-Helix Motif
11
Zinc Fingers
C2H2 C4 C6
http//www.web-books.com/MoBio/Free/Ch4F2.htm
12
Regulatory Protein in Yeast
13
Leu Zippers
14
Leu Zippers
15
DNA Replication
16
DNA Replication
17
DNA Replication is semidiscontinuous
18
DNA Damage
19
DNA Repair
20
DNA Repair
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