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Biotechnology

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Biotechnology Analysis of Clonal ... Applications of DNA Technology Disease diagnosis Gene Therapy Pharmaceutical products Forensic uses Environmental applications ... – PowerPoint PPT presentation

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Title: Biotechnology


1
Biotechnology
  1. Analysis of Clonal DNA
  2. DNA Cloning
  3. Stem Cells
  4. Practical Applications of DNA Technology

2
DNA Cloning Recombinant DNA
  • Major tool-restriction enzymes discovered in
    late 1960s, cut DNA at restriction site, highly
    specific, manufactured by bacteria
  • Cloning vector-carrier for moving DNA into a cell
  • http//www.wehi.edu.au/education/wehitv/restrictio
    n_enzyme_ecor1/

3
Procedure for cloning a eukaryotic gene in a
bacterial plasmid
4
How to get a eukaryotic gene to function in a
prokaryote
  • Basic problem introns!!
  • Solution production of cDNA
  • (artificially produced DNA that is made by using
    reverse transcriptase on a mRNA)

5
DNA Libraries
  • DNA can be cut with restriction enzymes and
    inserted into plasmids, inserted into bacteria,
    and stored whole genome is maintained
  • cDNA libraries eliminate everything except the
    genes

6
PCR-polymerase chain reaction
  • Rapid amplification of a piece of DNA
  • Billions of copies in a few hours
  • Used by CSI,
  • Jurassic Park?
  • Prenatal diagnosis

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8
Gel Electrophoresis
  • Produces DNA fingerprints
  • Separates RFLPs by using an electrical field
  • Can be used to identify some genetic diseases
  • Presence of a particular gene
  • Used as genetic markers

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11
Other Technologies
  • Hybridization
  • Human Genome Project
  • Physical mapping
  • Sequencing DNA
  • Genome Analysis (DNA sequences, gene expression,
    and gene function)

12
Cloning Plants
13
Cloning Animals-nuclear transplantation
14
Stem Cells
  • Unspecialized cell that can reproduce and
    differentiate
  • Types of stem cells
  • Embryonic stem cells from blastula
  • Adult stem cells

15
Practical Applications of DNA Technology
  • Disease diagnosis
  • Gene Therapy
  • Pharmaceutical products
  • Forensic uses
  • Environmental applications
  • Agricultural applications

16
iRNA
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33
Videos and Websites
  • http//www.pbs.org/wgbh/nova/sciencenow/0302/04.ht
    ml
  • http//www.geneticorigins.org/mito/mitoframeset.ht
    m
  • http//genomics.energy.gov/
  • http//www.dnalc.org/ddnalc/resources/cycseq.html
  • http//www.sumanasinc.com/webcontent/animations/co
    ntent/pcr.html
  • http//www.sumanasinc.com/webcontent/animations/co
    ntent/plasmidcloning.html
  • http//www.ncbi.nlm.nih.gov/Genbank/index.html

34
Websites and Videos
  • http//www.actionbioscience.org/biotech/pecorino2.
    html
  • http//www.dnai.org/index.htm
  • http//people.ibest.uidaho.edu/etop/vgp/index.htm
    l
  • http//www.dnalc.org/resources/animations/restrict
    ion.html
  • http//www.hhmi.org/biointeractive/dna/animations.
    html
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