The comparing of natural antibacterial substance on E. Coli

1
The comparing of natural antibacterial substance
on E. Coli

• Manhasset Science Research
• Jean Cheng Period 4

2
Need
Figure 1 Antimicrobial drugs resistance pattern
of urinary E. coli from Southern Brazil
http//www.scielo.br/img/revistas/bjm/v39n4/arq27_
fig01.jpg
3
Knowledge Base
Figure 2 Chemical formula for Penicillin http//i
tech.dickinson.edu/chemistry/wp-content/uploads/20
08/04/penicillin-core1.png
4
Knowledge Base
Figure 2 Chemical formula for Penicillin http//i
tech.dickinson.edu/chemistry/wp-content/uploads/20
08/04/penicillin-core1.png
5
Knowledge Base (cont)
Figure3 Penicillin attacking the peptidocglycan
http//upload.wikimedia.org/wikipedia/commons/thum
b/8/8b/Gram_negative_cell_wall.svg/800px-Gram_nega
tive_cell_wall.svg.png
6
Knowledge Base (cont)
Figure3 Penicillin attacking the peptidocglycan
http//upload.wikimedia.org/wikipedia/commons/thum
b/8/8b/Gram_negative_cell_wall.svg/800px-Gram_nega
tive_cell_wall.svg.png
7
Knowledge Base (cont)
Figure 4 Tea trees terpene chemical
formula http//www.wacker.com/cms/appL/mdm_images/
R67_IV-1_-432011277_8959.jpg
Figure 5 Pectin Chemical formula http//sci-toys.
com/ingredients/pectin_2.gif
8
Knowledge Base (cont)
Figure 6 Aloe vera http//apps.who.int/medicinedo
cs/fr/d/Js2200e/5.html
9
Knowledge Base (cont)
Figure 7 Citral http//www.impgc.com/images/Struc
tures/Citral.bmp
10
Literature Review
Figure 8Chemical Change in Allicin subjected to
different conditions. (Ariga, et al.
2008) http//media-2.web.britannica.com/eb-media/3
3/7433-004-96DFD197.gif
11
Literature Review (cont)
Figure 9 Antimicrobial activity of aloe vera gel
(Alemdar, et al. 2009)
12
Literature Review (cont)
Figure 10Yuzu fruit seed oil on different
microbial (Nakazono, et al. 2006)
13
Literature Review (cont)
Figure 1112 different essential oil on different
species of bacteria. (Rusenova, et al. 2009)
14
Purpose

• Find which natural substance is best in
  inhibiting E. coli.
• Null Hypothesis No effect
• Alternative Hypothesis Tea tree oil is the best
  antimicrobial substance against E. coli

15
Methodology
The comparing of natural antibacterial substance
on E. Coli
Essential oil and antibiotic plant substance N12
Aloe (anthraquinones) N3
Garlic (allicin) N3
Tea Tree oil N3
Yuzu fruit oil N3
Petri dish, E.coli, filter paper, glass spreader
Disc diffusion assay method
Millimeter
SPSS ANOVA Test
16
Protocols
Paper disk with substance being tested
Bacteria on agar
Inhibited Zone(mm)
Figure 12 Disk Diffusion method http//diverge.hu
nter.cuny.edu/weigang/Images/20-17_diskdiffusion.
jpg
17
Protocols (cont)
Figure 13 http//gsbs.utmb.edu/microbook/images/f
ig10_5.jpg
18
Do-Ability

• Not expensive
• doesnt require large amount of space
• Some materials found in lab or supermarket
• E. coli is needed as well as agar, aloe, tea
  tree, yuzu, and garlic
• Natural antiseptic doesnt have negative effect

19
Budget
20
Bibliography

• Aiello, Castoki. Consumer Antibacterial Soaps
  Effective or Just Risky?. The Infectious Disease
  Society of America Journal. Vol 10, p1058-1078,
  2007
• Aljady, A. Biochemical Study on The Efficacy of
  Malaysian Honey on Inflicted Wounds An Animal
  Model. Medical Journal of Islamic Academy of
  Sciences. Vol 13 3. pg 125-132, 2000
• American Chemical Society. "Love That Garlic?
  Fresh May Be Healthier Than Bottled."
  ScienceDaily 12 June 2008. 7 May 2009
  lthttp//www.sciencedaily.com /releases/2008/06/08
  0609092230.htmgt.
• Amagase, H., Intake of garlic and its bioactive
  components. Nutritional Journal. Vol131(3S) pg
  955S-962S, 2001
• "antiseptic." Grolier Multimedia Encyclopedia.
  2009. Grolier Online. 3 May 2009
  lthttp//gme.grolier.com/cgi-bin/article?assetid00
  13590-0gt.
• Avallone, Eugene A., ed. "Antibiotic."
  McGraw-Hill Encyclopedia of Science and
  Technology. 8th ed. 20 vols. United States of
  America Library of Congress, 1960. 756-67.
• Brunner, Nasco. Do Bioactive Glasses Convey A
  Disinfecting Mechanism Beyond A Mere Increase in
  pH?. Internation Endodontic Journal, Vol 41(8)
  p670-678, 2008
• Carr, Anitra. Therapeutic Properties of New
  Zealand and Australian Tea Trees. New Zealand
  Journal, Vol 182, pg 47-51. 2009
• Chen, Tao. Triclocarban Enhances Testoterone
  Action a New Type of Endocrine Disruptor?.
  Endocrinology journal. Vol 1. p 10-27, 2007
• Cuttle, Leila. The efficacy of Aloe vera, tea
  tree oil and saliva as fist aid treatment for
  partial thickness burn injuries. Elsevier
  Journal. Vol 1. pg 1176-1182. 2008
• Davis. D. "Antibacterial Chemical Disrupts
  Hormone Activities, Study Finds." ScienceDaily 8
  December 2007. 3 May 2009 lthttp//www.sciencedaily
  .com /releases/2007/12/071207150713.htmgt.

21
Bibliography (cont)

• Freeman, Frachesco. Garlic chemistry stability
  of s-(2-propenyl)-2-propene-1-sulfinothioate
  (allicin) in blood, solvents, and simulated
  physiological fluids. J. Agric. Food Chem.
  Journal. Vol 43 pg2332-2338, 1995.
• Fulder, Stephen. Garlic nature's original
  remedy. 2nd ed. United States Inner Traditions /
  Bear Company, 2000.
• Ingram, Cass. Tea Tree Oil The Natural
  Antiseptic. 1st ed. United States Knowledge
  House, 1999.
• Koizum. Love That Garlic? Fresh May Be Healthier
  Than Bottled Diallyl sulfides in garlic
  activate both TRPA1 and TRPV1. Biochemical and
  biophysical research communications journal. Vol
  19. p929-934, 2009
• Lawson L.D, Characterization of the formation of
  allicin and other thiosulfinates from garlic.
  Planta Medical Journal. Vol 58. pg345-350, 1992.
• Macpherson, Casey. The Pungency of Garlic
  Actiation of TRPA1 and TRPV1 in Response to
  Allicin. Current Biology Journal. Vol 15,
  p929-934, 2005
• Nagane, N. Efficacy of Topical Honey Therapy
  Against Silver Sulphadizine Treatment in Burns A
  biochemical study.Indian Journal of Clinical
  Biochemistry,Vol 192. pg 173-176. 2004
• Nakazono, G. Studies on Antimicrobial and
  Antioxidative Substance of Yuzu (Citrus junos
  hort. ex Tanaka) Seed. Journal of Biological
  Sciences. Vol 1. p 135-139, 2006
• Rusenova, Ishiwa. Antimicrobial Activities of
  Twelve Essential Oils Against Microorganisms of
  Veterinary Importance. Trankia Journal of
  Sciences. Vol 7. p37-43, 2009

22
Bibliography (cont)

• Shashikanth, K.N. Effect of feeding raw and
  boiled garlic (Allium sativum l.) on the growth,
  caecal microflora and serum proteins of
  albino rats. Nutrition Reports Int. Journal.
  Vol33(2) pg 313-319. 1986
• Society for General Microbiology. "Tea Tree Oil
  And Silver Together Make More Effective
  Antiseptics." ScienceDaily 6 April 2009. 3 May
  2009 lthttp//www.sciencedaily.com
  /releases/2009/03/090329205453.htmgt.
• Tao. Cho. Chemical Composition of Essential Oil
  From The Peel of Satsuma Mandarin. Journal of
  Natural Sciences. Vol 7(9), p1261-1264,2008