??-?? ??? ??? ?? : ??? ??? (specificity), ? (amount), ??? (affinity) - PowerPoint PPT Presentation

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??-?? ??? ??? ?? : ??? ??? (specificity), ? (amount), ??? (affinity)

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Title: ??-?? ??? ??? ?? : ??? ??? (specificity), ? (amount), ??? (affinity)


1
14. ??-?? ??? ??? ???
  • ??-?? ??? ??? ?? ??? ??? (specificity), ?
    (amount), ??? (affinity)
  • ??? ???? ??? ???? ??? ? ?? ??? ????? ??? ??
  • ??-?? ??? ???? ???? ?? ??? ???? ?? ??, ??? ?? ??
    ?? ??? ??? ? ??.
  • ??? ??? ??? ??? ???? ???? ?????? ???? ??
    (serological assay)??? ??, ??? ???? ??? ???
    (serology)???? ??.

2
14. ??-?? ??? ??? ???
  • ??? ??????? (Affinity Chromatography) ??? ?????
    ??? ? ?? ?? (beads)? ????? ?? ?, ? ???? ??? ?
    (column)? ???. ??? ???? ? ??? ????? ?? ??? ???
    ????. ?? pH? 2.5 ?? ?? 11 ??? ???? ????? ??? ???.
  • ? ??? ???? ?? ?? ???? ??? ? ? ??.

3
14. ??-?? ??? ??? ???
  • ??????? (Radioimmunoassay. RIA) ? ???????
    (Enzyme-Linked Immunosorbent Assay, ELISA)
  • ?? ?? ??? ?? ??? ??? ???? ???? ???? ??? ?? ???
    (ELISA? HIV ?? ?? ? ??? ??? ????, RIA? ??? ??? ??
    ????)
  • ??? ?? ??? ??? ??? ?? (125I) ?? ??? ???? ?, ???
    ??? ???? ?? ?? (multi-well plate)? ?????
  • ?? ??? ??? ???? ??? ??? ?? ???? ????? ??? ???? ??
    ??? ????.

4
14. ??-?? ??? ??? ???
  • ???? (Agglutination Test)
  • ??? ??? ??? ??? ?? ??,
  • ??? ? ??? ??? ????
  • ??? ??? ????
  • ??? ???? ???? ????
  • ??? ??? ?? ?? ???? ???
  • ???? ????? ???.

5
14. ??-?? ??? ??? ???
  • ???? (Precipitation Reaction)
  • ??? ?? ??? ???? ??? ????? ??? ??, ??? ?? ????
    ??? ???? ???? ?? ??? ???? ??
  • ??? ??? (???? ???, antigen-antibody complex)? ???
    ??? ? ?? ??.

6
14. ??-?? ??? ??? ???
  • Coombs Test
  • Rh-? ???? Rh? ??? ???? ?? ??? Rh ? ?? ???
    ???, ? ??? ??? ??? ???? ??, ???? ??? ? ? ??.
  • ??? ???? ??? ??? ???? ?, ??? ?? ????? ?? ???
    ????? ??? ??? ??? (Direct Coombs test)
  • ??? ??? ???? ???? ?, ?? ? ?????? ??? ????? ????
    ??? (Indirect Coombs Test)

7
14. ??-?? ??? ??? ???
  • ????? (Monoclonal Antibody)
  • ????? ??? ??? ?? (B cell, Plasma cell ? ??)? ???
    ???? ??? ?? ?????? ????
  • ?? ?, ???? ?? ????? ?????? ???? ????(hybridoma)?
    ???? ?
  • ??? ??? ???? ????? ??? ?, ??? ???? ????
  • ??? ????? ??? B??? ??? ??(clone)??? ??? ??? ? ??
    ??????? ???. ??? ??? ??????? ??.
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