Grind tissue in liquid N2 - PowerPoint PPT Presentation

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Grind tissue in liquid N2

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7 ml Trizol/g tissue (use glass pipettes) Grind tissue in liquid N2 vortex Thaw on ice Shake for 5m _at_ RT Hand shake for 15s _at_ RT Incubate _at_ RT X 5m – PowerPoint PPT presentation

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