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Wastewater Treatment Plants

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Wastewater Treatment Plants & Bacteria Strategies for Compliance Best Practices for Effluent Sampling TANNY BUSBY & LAURA BONJONIA ENVIRODYNE LABORATORIES, INC. – PowerPoint PPT presentation

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Title: Wastewater Treatment Plants


1
Wastewater Treatment Plants Bacteria Strategies
for Compliance
  • Best Practices for Effluent Sampling

TANNY BUSBY LAURA BONJONIA ENVIRODYNE
LABORATORIES, INC. April 26, 2011
2
Where Does the Analytical Testing Begin?
  • Container Preparation
  • Sample Collection
  • Chain of Custody
  • Preservatives
  • Field QC

3
What is a Representative Sample?
Best Practices for Effluent Sampling
  • Definition
  • Every Member of the Population has an Equal
    Chance of being Selected

Typically a Population is very large
A Sample is a Subset of the Population
4
(No Transcript)
5
The sample reflect characteristics of the
Effluent
Best Practices for Effluent Sampling
6
Identification of Sampling Points
Best Practices for Effluent Sampling
  • Effluents
  • Influents
  • Process Control
  • Collection Systems

7
DEFENSIBLE DATASample Collection
  • Grab
  • e-Coli
  • Fecal Coliform
  • Oil Grease
  • DO
  • pH
  • Cyanide
  • VOC
  • Chlorine Residual
  • Composite
  • Metals
  • BNA
  • Pesticides
  • TPH
  • Minerals
  • Nutrients (Nitrogen)
  • BOD
  • TSS (Solids)

8
DEFENSIBLE DATAQUALITY CONTROL
  • Calibrate Field Equipment - (Document in field
    log) pH, DO Cl2 Meters
  • Field Blanks
  • Trip Blanks
  • Rinseates
  • Field and Lab Duplicates
  • Field and Lab Matrix Spikes (MS)
  • Laboratory control samples (LCS)
  • Method Numbers

9
WHAT ARE COLIFORM
  • Gram stain
  • Gram negative (turns red)
  • Do not produce spores
  • Bacilli (rod shaped)
  • Aerobic (require oxygen) or Facultative Anaerobic

10
WHERE ARE COLIFORM(s) FOUND?
  • Surface Water
  • Decaying Plants
  • Soil
  • Intestinal Tract of Warm-blooded Animals

11
TYPES OF COLIFORM BACTERIA
12
Microbes Commonly Regulated in Wastewater
Effluents
  • Fecal Coliform
  • 200 CFU/100ml Monthly Geo-Mean (Avg.)
  • 400 CFU/100ml 7 Day Geo-Mean (Avg.)
  • 800 CFU/100ml Single Grab Max.
  • E. coli (Escherichia coli)
  • 126 CFU/100ml Monthly Geo-Mean (Avg.)
  • 394 CFU/100ml Single Grab Max.
  • Enterococci
  • 36 CFU/100ml Monthly Geo-Mean (Avg.)

13
COLIFORM LAB TESTING METHODS
  • Multiple Tube Fermentation (Quantifies)
  • Reported as MPN (most probable number)
  • Membrane Filtration (Quantifies)
  • Reported as CFUs/100 mL (colony forming units)
  • Quanti-tray (Quantifies)
  • Colilert 18-24 hours
  • Reported as MPN
  • Positive sample turns yellow E.coli fluorescence
  • Presence/Absence (Qualifies)
  • Colilert, Colisure
  • Positive sample turns yellow (Colilert) turns
    magenta (Colisure)
  • E.coli fluorescence

14
E. coli
  • Membrane filtration
  • m-ColiBlue 24
  • Total coliform and
  • E. coli simultaneously
  • E. coli Blue colonies
  • Total coliform Red or blue colonies
  • Other methods are approved

15
ENTEROCOCCI PLATE
  • Membrane filtration
  • SM 9230 C
  • CFUs/100 ml
  • Blue colonies
  • Other methods
  • Enterolert
  • MPN (Most Probable Number)

16
COLILERT- QUANTITRAY
Incubate 18-24 hours- Most Probable Number
TOTAL COLIFORMS
E.Coli (UV Light)
17
SAMPLING HOLD TIMES
  • Fecal Coliform, E. coli and Enterococci
  • Grab (Not Composite)
  • Sterile container
  • Hold Time Not to Exceed a Total of 8Hrs
  • 6 hours transport 2 hours set up

18
Micro Samples How to Collect Effluent
  • Directly into Sterile Testing Container

19
How to Collect and Split Samples
20
Total Coliform Qualitative Analysis
(PRESENCEABSENCE)
E.coli positive (UV Light)
  • COLILERT-18
  • Incubate 18-22 hours
  • COLILERT
  • -Incubate 24-28 hours

COLISURE -Incubate 24-48 hours
21
QUESTIONS
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