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The survey of genetically modified foods on the Hungarian market, with method development and adaptation* Official Control of the Genetically Modified Foods in Hungary

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Title: The survey of genetically modified foods on the Hungarian market, with method development and adaptation* Official Control of the Genetically Modified Foods in Hungary


1
The survey of genetically modified foods on the
Hungarian market, with method development and
adaptationOfficial Control of the Genetically
Modified Foodsin Hungary
  • Fodor József National Center for Public Health
  • NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION

There were only a few similar surveys performed
in the EU.
2
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • The INSTITUTE

Director Dr. Imre Rodler
3
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
 
Department of Food Additives and Chemical
Contaminants
Department of Food Chemical Analysis
Department of Nutrition Hygiene
Department of Food Microbiology

Division of Food Additives and Packaging
Additives
Division of Protein and Vitamin
Division of Nutrition Physiology
Division of Bacteriology
Division of Chemical Contaminants
Division of Metal Analysis and Lipid
Division of Nutrition Biochemistry
Division of Microbiological Biochemistry
Veterinary Drug Residues Group
Division of Food Product Registration
Division of Public Nutrition and Therapeatic
Nutr.
Entomology Laboratory
  Cosmetic Group
Mycotoxicology Laboratory
Laboratory of Genetically Modified Organisms
4
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • Tasks

  • participates in the preparation of legistlation,
    with especial regard to the determination
  • of maximum residue limits for foods
  • regularly supervises the food microbiological and
    chemical limits and makes proposal
  • for their amendment
  • performs the comprehensive microbiological, GMO,
    chemical, toxicological and
  • parasitological laboratory analysis of foods.
  • performs licencing activity with regard to foods
    meeting particular nutritional demands,
  • nutriments, new food additives, new food
    packaging materials and cosmetics, and
  • authorizes the use of cleaning agents and
    disinfectants in food industry
  • issues certficates attesting that imported foods
    to be put on the market conform to the
  • public health requirements
  • issues expert opinion for the manufacture of
    so-called new foods and foods treated by
  • irradiation, and for the licencing of veterinary
    medicinal products and pesticeds
  • provides technical guidance and consulting to
    assist the work of the NPHMOS in the
  • field
  • performs nutrition-physiological and nutritional
    health studies and conducts consumer
  • awarness campaigns.

5
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • GMO related performance

  • In 1998 the XXVII.Act on gene technology came
    into force in Hungary,
  • and one year later the Regulation of the Minister
    of Agriculture
  • (1/1999. (I.14.)) was published on the execution
    of the Act, which
  • regulates also the labelling of GM foods. The
    Hungarian regulation on
  • GMO is conform with that of the European Union.
  • In accordance of the Regulation 1/1999. (I.14.) a
    laboratory for the
  • official control of GM foods was established in
    the National
  • Institute of Food Hygiene and Nutrition.
  • GMO screening and quantification is carried out
    in our GMO laboratory in
  • the case of meat products and foods from plant
    origin.

6
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • Dedicated to the official GMO control in the case
    of meat industry products and products of plant
    origin
  • (Annex 5 of the FVM regulation No 1/1999. (I.
    14.))

Fodor József National Centre for Public Health -
National Institute of Food Hygiene and Nutrition
GMO Laboratory Address 1097 Budapest, Gyáli út
3/A Postal address 1476 Budapest 100, P.O.Box
52
7
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • The GMO Laboratory

8
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • Instuments
  • GeneAmp5700 Primus 96 plus
  • ? quantification ? detection

9
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • GMO team
  • Boldizsár Vajda
  • Head of GMO Lab
  • Zsuzsanna Bátki
  • Bioengineer Szilvia Baranyi
  • Assistant

10
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • Steps of PCR based detection
  • 1.    DNA isolation
  • 2.    GMO screening conventional PCR method,
    followed by
  • agarose-gelelectrophoresis  and
  • restriction digestion of the GMO-positive
    amplicon
  • 3.    GMO quantification real-time PCR method

11
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • Primers
  • Control primers
  • Invertase and zein gene specific primer (in the
    case of MAIZE)
  • Lectin gene specific primer (in the case of SOY)
  • Chloroplast specific primer (for plants)
  • GMO specific primers
  • 35S promoter
  • NOS terminator
  • Specific primers
  • MON810 specific primer (for YieldGard maize)
  • RR specific primer (for RoundupReady soybean)
  • PG gene specific primer (for FlavrSavr tomato)
  • Hpt gene specific primer (for GM potato)

12
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • The PROJECT

In the framework of Technical Research and
Development projects BIOTECHNOLOGY 2002
Funded by the Ministry of Education Research and
Development
13
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • Aim of the project
  • Investigate the presence of genetically modified
    components in foods on the Hungarian market
  • Determine the quantity of genetically modified
    components in foods on the Hungarian market

14
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • Samples
  • Type of samples
  • Raw materials
  • Flour? Bakery
  • Snacks
  • Dry foods ? Powders
  • Meat products
  • Sauces
  • Drinks
  • Other
  • Foods containing
  • Soy (100 samples)
  • Maize (100 samples)
  • Soy and Maize (50 samples)
  • Tomato (50 samples)
  • Potato (50 samples)

15
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • GMO screen and quantification


Maize products
Soy products
Tomato products
Potato products
16
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • Analysis of the samples
  • Screening for
  • 35S promoter
  • NOS terminator
  • Determination of the GM trait
  • In the case of GMO positive samples
  • Quantification
  • In the case of GMO positive soy or maize

17
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • Expected results
  • Information about the the presence and quantity
    of
  • GMOs on the market
  • Basis of information of consumers
  • Background of the activities of authorities
  • Reliable laboratory background of GMO control
  • Accredited GMO laboratory for the official
    control of
  • GM foods

18
Fodor József National Center for Public Health
NATIONAL INSTITUTE OF FOOD HYGIENE AND NUTRITION
  • - THANK YOU -

Contact person Dr. Boldizsár P. Vajda Head
of GMO Laboratory E-mail vaj4616_at_ella.hu
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