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The Basics of Quality Control for Insect Rearing 2013

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Title: The Basics of Quality Control for Insect Rearing 2013


1
The Basics of Quality Control for Insect
Rearing 2013
  • Norm Leppla
  • University of Florida
  • Department of Entomology
  • and Nematology

2
Norms Career in Insect Rearing
I. Introduction
  • University of Arizona- Insect rearing research, 2
    years
  • USDA, ARS- Insectary management, Florida and
    Texas, 17 years
  • USDA, APHIS- Methods development, Washington DC
    and international, 7 years
  • University of Florida- Integrated pest management
    and Biological Control, 16 years
  • Chemical ecology and quality of Spalangia
    cameroni (Erika Machtinger)
  • Rearing and quality of Tamarixia radiata (Chris
    Kerr)

3
Countries of Workshop Students
4
Quality Control Topics
  • Introduction- Learning how to rear quality
    insects
  • Colony Establishment and Maintenance
  • Monitoring Quality
  • Evaluation and Management
  • Guidelines for Quality Systems
  • Total Quality Management

5
Learning How to Rear High Quality Insects
  • Apprentice in an insectary
  • Network with other professionals
  • Literature plus trial-and-error
  • Visit other insectaries
  • Reviews by experts
  • Education and Training programs

6
MSU Insect Rearing Workshop
Frank Davis
John Schneider
7
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8
Online Rearing Courses- 2013
  • An Introduction to Insect Rearing
  • Physical Aspects of Insect Rearing
  • Biological Aspects of Insect Rearing
  • Following Insectary SOPs How and Why?

2004
http//insectdiets.com/
9
Insectary Manager Network
  • Anyone associated with the mass rearing of insects

65 Members
http//www.entsoc.org/
10
Insect Rearing Professionals Google Group
Subscribe (leonwesterd_at_gmail.com)
Léon Westerd Group moderator Head of insect
rearing Wageningen UR, Netherlands
11
VI. Total Quality Management
12
Parts of an Insect Rearing System
13
Generic Guidelines for Quality Systems
  • Policy, Planning and Administration
  • Design Assurance and Change Control
  • Control of Purchased Materials
  • Production Quality Control
  • User Contact and Field Performance
  • Corrective Action
  • Employees- Select, Train and Motivate

14
A Complete QA System
15
Quality Assurance Actions Frequency of Actions Frequency of Actions Frequency of Actions
Production and Process Control Routinely Periodically Possibly
Have up-to-date standard operating procedures   X  
Use check sheets to monitor rearing processes X    
Record daily production data X    
Track changes in production X    
Review production with staff X    
Improve product quality based on staff feedback   X  
Product Control      
Conduct product quality control assessments X    
Have established minimum thresholds of quality X    
Use standard shipping and handling procedures X    
Customer Service      
Add customer feedback forms to shipments X    
Add product use instructions to shipments X    
Have an established customer service program X    
Improve product quality based on customer feedback   X  
16
Quality Assurance Actions Frequency of Actions Frequency of Actions Frequency of Actions
Research Routinely Periodically Possibly
Maintain internal methods improvement     X
Conduct publishable research     X
Communicate research needs to outside researchers   X  
Collaborate with researchers without funding projects     X
Contract for research and provide funding     X
Outreach      
Provide materials for training activities   X  
Provide products for training activities   X  
Participate in training activities   X  
Describe quality assurance program   X  
17
II. A. Purposes for Rearing Insects II. B. Types
of Insect Rearing Systems II. C. Options for
Colonizing Insects II. D. Maintaining the Quality
of Colonized Insects
II. Colony Establishment and Maintenance
18
II. A. Purposes for Rearing Insects
19
White witch, Thysania agrippina (Lepidoptera
Noctuidae
20
Small-Scale Rearing Butterflies
II. B. Types of Insect Rearing Systems
Painted Lady
Monarch
21
Annie Lorie
Insect rearing is never a boring task insects
are always doing something interesting and pose
new challenges all the time
Punky Rogers
Medium-Scale Rearing
22
Mass Rearing- Medfly
El Pino, Guatemala
Capacity- 3.5 billion sterile male pupae per
week
Metapa, Mexico
23
Honolulu, Hawaii
Nori Tanaka
24
II. C. Options for Colonizing Insects
Peter Ebling Insect Producer Database Mgr. Great
Lakes Forestry Centre 1219 Queen St. East Sault
Ste. Marie, ON P6A 2E5 peter.ebling_at_nrcan.gc.ca (7
05) 541-5517
25
  • World-wide listing, 35 insect 21 nematode
    orders
  • Data submitted by sources
  • Searchable database
  • Expand clients
  • Adopt-a-colony

http//www.insect.glfc.cfs.nrcan.gc.ca
26
Field Collecting Cabbage Loopers
27
Continuous Improvement of Insect Rearing
Capability
28
II. D. Maintaining the Quality of Colonized
Insects
29
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30
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31
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32
Maintaining the Quality of Colonized Insects
  • Collection of appropriate biotypes
  • Colonization and strain development
  • Rearing proficiency
  • Colony management
  • Optimization
  • Strain replacement

33
III. A. Quality Control Criteria and
Standardized Tests III. B. Sampling for
Consistent Quality III. C. Production,
Process and Product Control
III. Monitoring Quality
34
III. A. Quality Control Criteria and Standardized
Tests
  • Quantity- Number of Pupae or Adults
  • Size- Weight of Pupae
  • Fecundity- Oviposition and Egg Hatch
  • Rate of Development-Synchronization
  • Adult Behavior-Flight, Longevity
  • Field Performance- Achieve Purpose

35
Specifications- Requirements for a product or
service
  • Standards- The level of quality at which a
    specification is written

36
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37
III. B. Sampling for Consistent Quality
  • Count Measurement N
  • Mean Sum/Number of Counts X
  • Variance Sum of (N X)2 / n-1 2
  • Standard Deviation Square Root of 2 SD

38
Number of Cabbage Looper Pupae Per Rearing
Container
Container 1 2 3 4 5 6
1 175 157.6 17.4 302.76 190 190 191 180 142
2 167 157.6 9.4 88.36 191 191 190 180 176
3 144 157.6 -13.6 184.96 191 190 192 189 156
4 166 157.6 8.4 70.56 191 192 190 176 138
5 136 157.6 -21.6 466.56 190 191 190 174 181
Average 157.6 190.6 190.8 190.6 179.8 158.6
Sum of Squares 1,113.2
Variance 278.3
Standard Deviation 16.7 0.5 0.8 0.9 5.8 19.4
Shelf 1 is lowest
39
Quality Control Charts
40
III. C. Production, Process and Product Control
41
IV. A. Structured Diagnostic
Procedures IV. B. Quality Control versus
Methods Improvement IV. C. Periodic Review
IV. Evaluation and Management
42
IV. A. Structured Diagnostic Procedures
43
IV. B. Quality Control Versus Methods Improvement
  • Quality Control
  • Monitor indicator variables
  • Evaluate multiple variables
  • Troubleshoot using QC data
  • Conduct evaluations rapidly
  • Goal is to restore stable production
  • Methods Improvement
  • Conduct evaluations methodically
  • Test one variable at a time
  • Use controlled experiments
  • Test results in the production system
  • Goal is to optimize production

44
Type of Research Research Priorities
Feasibility/market analysis Natural enemy effectiveness in controlling target pests Product development Quality assurance systems
Mass production   Rearing facilities Mechanized equipment Rearing materials Natural and artificial diets Rearing and harvesting techniques Production quality control Process quality control Product quality control
Utilization   Product handling and storage Automated counting technology Improved packaging and shipping Extended use period Efficient scouting techniques Optimal release numbers, rates and timing Mechanized application technologies Efficient product evaluation procedures Utilization quality control
Application environment Site-specific pest prevention Systems to maximize the effectiveness of natural enemies Natural enemy use with pesticides Systems with multiple products and pests Effectiveness in seasonal and perennial crops
45
Olivefly Methods Improvement
Bactrocera oleae
FAO/IAEA Entomology Unit Seibersdorf, Austria -
2009
46
Eggs hatched
Eggs laid
Eggs/Cage
Sex ratio malefemale
47
Eggs laid
Eggs Hatched
Total eggs laid
Number of females
48
Eggs laid
Eggs hatched
Total eggs laid
Number of males
49
Olivefly Methods Improvement
50
IV. C. Periodic Review
51
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52
V. Guidelines for Quality Systems
IOBC
53
Global Quality Control Programs
  • Individual Companies Customers
  • International Standards ISO 9000 IOBC
    Guidelines
  • The Marketplace Quality Products

54
International Organization for Biological Control
to facilitate and advance cost-effective rearing
of high quality insects and other arthropods in
support of biological control and integrated pest
management
Mission
http//www.amrqc.org
55
Workshops of the IOBC, WGQC (AMRQC)
  • 1982 Gainesville, Florida E. F. Boller and D.
    L. Chambers
  • 1984 Wadenswil, Switzerland E. F. Boller and D.
    L. Chambers
  • 1986 Guatemala City, Guatemala C. 0. Calkins
  • 1988 Vancouver, Canada C. 0. Calkins
  • 1991 Wageningen, Netherlands F. Bigler and J. C.
    van Lenteren
  • 1992 Horsholm, Denmark F. Bigler
  • 1993 Rimini, Italy M. Benuzzi and N. C. Leppla
  • 1995 Santa Barbara, California R. F. Luck and N.
    C. Leppla
  • 1998 Cali, Colombia N. C. Leppla and T. R.
    Ashley
  • 2003 Montpellier, France P. De Clercq, S.
    Grenier and NCL
  • 2007 Montreal, Canada S. Grenier
    and C. S. Glenister
  • 2010 Vienna, Austria P. De Clercq and T. A.
    Coudron
  • 2013 Bangalore, India P. De Clercq
    and T. A. Coudron

56
Insect Pest Control (IPC)
http//www-naweb.iaea.org/nafa/ipc/public/ipc-mode
l-plan-sterile-insect-technique.html
57
Lepplas Insect Rearing Quality Control References
  • Leppla, N. C. 2013. Quality Assurance for
    Mass-Reared Parasitoids and Predators. In Mass
    Production of Beneficial Organisms. Elsevier.
  • Leppla, N. C. 2009. The basics of quality control
    for insect rearing. In Principles and
    Procedures for Rearing Quality Insects.
    Mississippi State University.
  • Leppla, N. C. 2004, 2008. Rearing of Insects.
    Encyclopedia of Entomology. Kluwer.
  • Leppla, N. C. 2003. Guidelines for quality
    control of commercially produced natural
    enemies. In Quality Control and Production of
    Biological Control Agents, Theory and Testing
    Procedures. CABI.
  • Leppla, N. C. 2002, 2009. Rearing of Insects.
    Encyclopedia of Insects. Academic Press.

58
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