Diversity and Functional Variation of Denitrifying Bacterial Communities in the Cape Fear River Estuary - PowerPoint PPT Presentation

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Diversity and Functional Variation of Denitrifying Bacterial Communities in the Cape Fear River Estuary

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Diversity and Functional Variation of Denitrifying Bacterial Communities in the Cape Fear River Estuary Brian Shirey Marine Biology Project Goals Provide insight into ... – PowerPoint PPT presentation

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Title: Diversity and Functional Variation of Denitrifying Bacterial Communities in the Cape Fear River Estuary


1
Diversity and Functional Variation of
Denitrifying Bacterial Communities in the Cape
Fear River Estuary
  • Brian Shirey
  • Marine Biology

2
Project Goals
  • Provide insight into denitrifying bacterial
    community structure
  • Examine relationships between bacteria and their
    environment
  • Provide answers to questions

3
Whats The Big Deal About Bacteria?
  • The good
  • The bad
  • And the ugly

Salmonella typhimurium
4
Denitrifying Bacteria
  • Anaerobic
  • Utilize nitrate during respiration
  • Comprise over 50 different genera
  • Ubiquitous

5
Ecological Importance
  • Vital role in global nitrogen cycle

6
Ecological Importance cont.
  • Contribution to global warming
  • Production of greenhouse gasses

NO3- NO2- N2O
NO N2
Nitrogen oxides
7
Ecological Importance cont.
  • Effective in bioremediation
  • Reduction of nitrate entering ground and surface
    waters

8
Global Significance
  • Understanding the mechanisms of denitrification
    is critical to our understanding of the global
    nitrogen cycle
  • Applied methodologies promote a healthy,
    balanced, captive ecosystem

9
Experimental Design
  • Collect samples
  • Perform bacterial diversity and phylogenetic
    analysis
  • Perform community functionality analysis

10
Sediment Sampling
  • Sediments collected from 5 sites along the cape
    fear river estuary

11
(No Transcript)
12
Measuring Bacterial Diversity
  • DNA sequencing
  • Isolation of functional genes encoding for
    nitrate reductase
  • Comparative analysis
  • Along environmental gradients
  • T-RFLP analysis
  • Profile of community structure

13
Measuring Functional Diversity
  • Incubation experiments
  • Designed to monitor denitrifying activity

Control
NaCl
Nitrate
Anaerobic Enrichment Cultures
14
Research Hypothesis
  • Different components of a community will respond
    to different environmental inputs to maintain
    community functionality

15
Questions To Be Answered
  • Do denitrifying bacteria show the capability to
    readily adapt to sudden changes in their
    environment?
  • Is survivorship of denitrifying bacteria greatly
    reduced in sub optimal conditions?
  • To what degree do environmental conditions
    determine community structure?

16
Schedule
  • Phase 1
  • Sequencing all DNA, setting up and initialize
    incubation experiment
  • Phase 2
  • Completing incubation experiment and performing
    T-RFLP analysis
  • Phase 3
  • Analyze data, write paper

Phase 1
Phase 2
Phase 3
Jan. Feb. March April May June July Aug.
Sep. Oct. Nov. Dec.
17
Conclusion
  • Denitrifying bacteria have a tremendous impact on
    the biosphere
  • Our biosphere is fueled by an underlying
    microbial world
  • The global community and the microbial community
    are eternally linked
  • Bacterial community as a window into past,
    present, and future

18
QUESTIONS?
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