Summary of Bioreactor Design and Operation

1
Summary of Bioreactor Design and Operation

• Modified batch and continuous reactors
• - Chemostat with cell recycle
• Keep high cell mass concentration in the
  reactor.
• Production of biomass and low-value product
• - Fed-batch
• Maintain low substrate concentration
• Secondary metabolites, prevent catabolite
  repression
• - Multi-stage chemostat reactor
• Separate cell growth and product formation
• Secondary metabolites, genetically modified cell
  culture

2
Summary of Bioreactor Design and Operation

• Modified batch and continuous reactors
• - Chemostat with cell recycle

(qp0, kd 0, X00, Monod equation is applied)
3
Summary of Bioreactor Design and Operation

• Modified batch and continuous reactors
• - Fed-batch

(qp0, kd 0, Monod equation is applied)
4
Summary of Bioreactor Design and Operation

• Modified batch and continuous reactors
• - Multi-stage chemostat reactor

5
Summary of Bioreactor Design and Operation

• Scale-up/down
• - Geometric similarity
• - Dynamics similarity

• Sterilization
• - Probability of unsuccessful sterilization
• 1- P0(t) 1-1-e-kdtN0
• - Deactivation of components in media
• CC0e-kdt

6
Summary of Bioreactor Design and Operation

• Immobilized cell systems
• Advantages and disadvantages
• Operation consideration
• agitation and aeration, determination of
  volumetric mass transfer coefficient kLa,
• heat removal, foam, etc.