2005 Anatomic Pathology Exam Microscopic Slide Review

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2005 Anatomic Pathology ExamMicroscopic Slide
Review
Presented at the57th Annual Meeting of the
ACVP December 3, 2006 Tucson, AZ
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2005 Microscopic Slide Exam Reviewpreparing the
microscopic exam
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2005 Microscopic Slide Exam Reviewpreparing the
microscopic exam

• Nov/Dec- initial committee meeting _at_ annual ACVP

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2005 Microscopic Slide Exam Reviewpreparing the
microscopic exam

• Nov/Dec- initial committee meeting _at_ annual ACVP
• Mid-Apr- contributions to section leader

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2005 Microscopic Slide Exam Reviewpreparing the
microscopic exam

• Nov/Dec- initial committee meeting _at_ annual ACVP
• Mid-Apr- contributions to section leader
• Section leader prepares draft exam
• -matrix process/tissue/species

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2005 Microscopic Slide Exam Reviewpreparing the
microscopic exam

• May/Jun- 2nd committee meeting,
• review of draft exam, keys prepared

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2005 Microscopic Slide Exam Reviewpreparing the
microscopic exam

• May/Jun- 2nd committee meeting,
• review of draft exam, keys prepared
• 5. Mid-Aug- slides, exam questions completed

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2005 Microscopic Slide Exam Reviewpreparing the
microscopic exam

• May/Jun- 2nd committee meeting,
• review of draft exam
• 5. Mid-Aug- slides, exam questions completed
• 6. Sep- exam time

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2005 Microscopic Slide Exam Review

• 1. rat, brain, astrocytoma
• horse, artery, strongylosis
• dog, skin, ceruminous gland carcinoma
• dirofilariasis
• 4. dove, liver, amyloidosis
• sea lion, kidney, leptospirosis

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2005 Microscopic Slide Exam Review

1. ox, lung, atypical interstitial pneumonia
2. cat, intestine, panleukopenia FIP
3. marmoset, bone, fibrous osteodystrophy
4. dog, salivary gland, infarct
5. ox, spinal cord, fibrocartilaginous embolus

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2005 Microscopic Slide Exam Review

• cat, kidney, lily toxicosis
• dog, eye, phthisis bulbi
• rhesus, lung, SIV CMV
• walleye, skin, fibrosarcoma
• ox, heart, ionophore toxicosis

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2005 Microscopic Slide Exam Review

1. dog, bone, metastatic carcinoma
2. horse, placenta, aspergillosis
3. dog, liver, autoimmune hemolytic anemia
4. rat (panel), thoracic mass, hibernoma
5. ox (TEM), oral mucosa, MCF

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Case 7 Tissue from a cat.

• Histopathologic description (12 points)
• Morphologic diagnosis(es) (4 points)
• Cause(s) (4 points)

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Case 7 Tissue from a cat.

• Histopathologic description (12 points)
• Intestine
• Mucosa
• Blunting of villi
• Collapse of lamina propria
• Dilated crypts with necrotic debris
• Muscularis, serosa, mesentery
• Perivascular accumulations of PMNs, macrophages
  and fibrin
• O/C
• Logical and clear presentation of features

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Case 7 Tissue from a cat.

• Morphologic diagnosis(es) (4 points)
• Necrotizing enteritis
• Pyogranulomatous perivasculitis/peritonitis
• Cause(s) (4 points)
• Feline parvovirus (Feline panleukopenia virus)
• Feline infectious peritonitis virus

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Case 9 Tissue from a dog.

• Histopathologic description (14 points)
• Morphologic diagnosis(es) (6 points)

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Case 9 Tissue from a dog.

• Histopathologic description (14 points)
• Salivary gland (ID)
• Vasculature
• Thrombosis
• Acini
• Coagulative necrosis
• Inflammation
• Granulation tissue

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Case 9 Tissue from a dog.

• Histopathologic description (14 points)
• Salivary gland (ID)
• Septa
• Inflammation
• Hemorrhage
• Fibrosis
• Ducts
• Inflammation
• Necrosis
• Squamous metaplasia
• O/C
• No points allotted

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Case 9 Tissue from a dog.

• Morphologic diagnosis(es) (6 points)
• Necrotizing sialoadenitis with thrombosis
  (infarction) and squamous metaplasia

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Case 11 Tissue from a cat.

• Histopathologic description (13 points)
• Morphologic diagnosis(es) (4 points)
• Most likely cause (3 points)

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Case 11 Tissue from a cat.

• Histopathologic description (13 points)
• Distribution
• Tubular epithelium
• Degeneration
• Necrosis
• Regeneration

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Case 11 Tissue from a cat.

• Histopathologic description (13 points)
• Casts
• Granular
• Protein
• Mineralization
• O/C
• Logical and clear presentation of features

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Case 11 Tissue from a cat.

• Morphologic diagnosis(es) (4 points)
• Diffuse tubular necrosis with casts
• Most likely cause (3 points)
• Lily toxicosis
• Aminoglycoside antibiotic toxicosis

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Case 16 Tissue from a dog.

• Histopathologic description (14 points)
• Morphologic diagnosis(es) (6 points)

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Case 16 Tissue from a dog.

• Histopathologic description (14 points)
• Bone
• Neoplasm location, pattern, cell morphology,
  cell borders, cytoplasm, nuclear morphology,
  nucleoli, mitoses, anisokaryosis, necrosis
• Proliferation of new bone
• Resorption of trabecular bone

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Case 16 Tissue from a dog.

• Histopathologic description (14 points)
• Muscle degeneration and atrophy
• O/C
• Logical and clear presentation of features
• Primary vs. secondary processes

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Case 16 Tissue from a dog.

• Morphologic diagnosis(es) (6 points)
• Metastatic transitional cell carcinoma in bone
• Hyperostosis
• Muscular atrophy and degeneration

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Case 19 Tissue from a rat.

• Microscopic description (16 points)
• Morphologic diagnosis (4 points)

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Case 19 (panel) Tissue from a rat.

• Microscopic description (16 points)
• A. HE
• Description pattern, cell morphology, cell
  borders, cytoplasmic vacuoles (fat), nuclear
  morphology, multinucleated cells, nucleoli, no
  mitotic figures
• B. Oil-Red-O
• Positive cytoplasmic staining lipid

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Case 19 (panel) Tissue from a rat.

• Microscopic description (16 points)
• C. Mitochondrial uncoupling protein-1
  immunohistochemistry
• Reactivity indicates presence of numerous
  mitochondria

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Case 19 (panel) Tissue from a rat.

• Microscopic description (16 points)
• D. TEM
• Many mitochondria
• Some mitochondrial swelling
• Fat droplets
• Morphology consistent with brown fat

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Case 19 (panel) Tissue from a rat.

• Morphologic diagnosis (4 points)
• Hibernoma

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2005 Microscopic Slide Exam Reviewscoring
Proportion () Passed Mean Score
Range of Scores
2003 2004 2005 2006 2003 2004 2005 2006 2003 2004 2005 2006
45 72 47 61 57 63 57 62 71-37 82-11 75-31 83-7