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Research Proposal


Research Proposal Duean 064-5 Joy 104-9 Pim 155-2 Research Design Research Design Chapter 1 Introduction What are the effects of Thai honey on acne? – PowerPoint PPT presentation

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Title: Research Proposal

Research Proposal
  • Duean 064-5
  • Joy 104-9
  • Pim 155-2

Chapter 1 Introduction
What are the effects of Thai honey on acne?
Background and Settings
Honey is a global household natural sweetener.
It is cheap, easy to find, and non-toxic. Honey
has been used as sweetener, food, supplement,
wound healer and cosmetics. Honey has been
used to treat bacterial infections since the
ancient time. It was found that honey has a great
antimicrobial activity. Therefore, it was claimed
to have an ability of treating acne. Honey has
been used to treat acne and other skin infections
for thousands of years. However, there is not yet
any study showing the exact effects of Thai honey
on acne. This research will be done to study
the effects that Thai honey has on acne.
Problem Statement
Researches showed that honey is extremely
effective in treating almost all kinds of skin
condition. It was clear that honey has a great
antimicrobial activity. A research showed that
honey can kill Staphylococci, a very harmful
bacteria. But there is no research showing its
effects on Propionibacterium acne, the bacteria
that causes acne. Therefore, the ability of honey
to treat acne is still a myth. The problem is
What are the effects of honey on
Propionibacterium acne, the bacteria that causes
acne? Therefore, our research question is
What are the effects of Thai honey on acne?
- To test the ability of Thai honey for treating
acne - To test honeys effect on acne, acne scar,
and facial skin - To determine the most suitable
condition for honey to treat acne - To compare
the acne treating activity with other products
Acnes applied with honey will heal faster than
ones that werent applied due to the
antimicrobial properties of honey.
Definition of Terms
Honey Acne Propionibacterium acne Antimicrobial
activity Inflammation Subject
Limitations of the Study  
- Subjects with different types of skin may have
different result. - Honey can only treat acnes
which occurred by bacterial infections. -
Subjects may not give full cooperation with the
course. - Different honeys have different
antimicrobial activities.
Basic Assumptions
This research is to study the effects that Thai
honey has on acne. Due to the fact that acne
commonly occurs in teenagers, therefore our
subjects are selected to be people with acne in
the age between 15-25.
Chapter 2 Review of literature
P. E. Pochi. (1992). The Pathogenesis and
Treatment of ACNE. Annual Review of Medicine,
Vol. 41 187 -198
This research studied the pathogenesis and
treatment of acne. From the research, it
concluded that acne is caused by the abnormality
of the keratinizing epithelium on the follicle
which is caused by the secretion of sebum that
leads to the inflammation by Propionibacterium
acne. Treatment of acne can be done by using
drugs with antikeratinizing, antibacterial, and
antiseborrheic actions. From the research, we
knew that one important cause of acne is from the
inflammation by the bacteria Propionibacterium
acne. Therefore, it is likely that honey can be
used to treat acne because of its antibacterial
Allen, K. L., Molan P.C. Reid G.M. (1991). A
survey of the antibacterial activity of some New
Zealand honeys. Journal Of Pharmacy and
Pharmacology43(12), 817-822.
The research was done to test the antimicrobial
activity in honeys from different flowers. Honeys
from 26 different flower sources were tested. The
result was that different flowers give different
levels of antimicrobial activities. The bests are
honeys from manuka and vipers bugloss. From
the research, it is best to specify the source of
the honey being tested because they have
different antimicrobial activities. Therefore we
specify our honey to be Thai honey.
Chanchao C. (2009). Properties and antimicrobial
activity of Apis Dorsata honey From Thailand. Pak
J Med Sci, 25, 313-318.
This research studied the properties and
antimicrobial activity of Apis Dorsata honey From
Thailand. From the research, (25-100 (v/v))
dilutions of honey could inhibit the growth of S.
aureus. And also A. dorsata honey can inhibit the
growth of some bacteria. Therefore, it suggests
that honey could be used as an antimicrobial
agent. From the research, we can now be sure
that Thai honey has an antimicrobial activity and
can be used as antimicrobial agent which may be
able to treat acne.
(1996). A comparison of the efficacy and safety
of adapalene gel 0.1 and tretinoin gel 0.025 in
the treatment of acne vulgaris a multicenter
trial. Journal of the American Academy of
Dermatology 34(3), 482-485.
In this research, they studied the efficacy and
safety of two drugs in treating acne. They
studied by selecting 223 patients and applied the
test materials to the entire face daily, for a
period of 12 weeks. The results were obtained
before treatment and in weeks 2,4,8, and 12 after
treatment. From the research, we could use
similar methodology in collecting data but
instead of applying testing material (honey) to
the entire face, we apply it only on acne.
Chapter 3 Procedure
Research Design
We will have 2 sample groups one is the
experimental group, the subjects will apply honey
on acne everyday before bed and the other one is
the control group, the subjects will not be using
any treatment. The course will take 3
months. The number of acne of the subjects will
be counted regularly and compared to the previous
one. The data will then be calculated to the
percent of the disappearing of acne and compared
between the experimental and control group.
Subject selection
  • Subjects are randomly selected to be 80
    teenagers (40 males, 40 females) in KKU age
    between 15 and 25 who have acne.
  • Subjects who want to quit or did not fully
    cooperate throughout the course will become

Outcome measures
  • 40 subjects20 males, 20 females (experimental
    group) will be having acne treatment by honey for
    3 months while the other 40 (control group) will
    be having no treatment. The number of acne will
    be collected before the treatment and after 2, 4,
    8, and 12 weeks.

Condition of testing
The subjects of the experimental group are given
honey to be applied on the acne. All subjects are
prohibited to use any other treatment while
participating in the course.
Apply honey on acne and leave it for 15 minutes
then wash out with water . Do it every day before
Data analysis
The data will be obtained by counting the number
acne before the treatment and after 2, 4, 8, and
12 weeks. The data will then be calculated as
percentage difference of acne disappearing
between the honey and non-honey group of the
sample The samples percentage difference will
be converted to the populations percentage
difference using inferential statistics. Statist
ical significance test will be done to ensure
that the data obtained is correct. Software
used microsoft excel, SPSS
Chapter 4 Results
Predicted Results
From the research, we can predict the results
that in the sample that apply honey on acne
(experimental group, both male and female), acne
heals much faster than in the sample that dont
apply honey on acne (control group, both male and
female) due to the antimicrobial activity of Thai
Chapter 5 Discussion
Predicted Discussion
Thai honey has an antibacterial activity. It can
kill Propionibacterium acne. As a result, Thai
honey can treat acne that is caused by the
inflammation of Propionibacterium acne. From
the predicted result, we can conclude that Thai
honey has the ability to treat acne. Further
researches such as marketing researches can be
done in the future using this research as a base.
P. E. Pochi. (1992). The Pathogenesis and
Treatment of ACNE. Annual Review of Medicine,
Vol. 41 187 -198 http//
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