UPLC/Q-TOF MS

1
UPLC/Q-TOF MS

• ?????

2
ACQUITY UPLC

• ???????(Ultra Performance LC)?????????????,????HP
  LC??????,????????????????????????????,????????????
  ???????

3
???HPLC????

• ??HPLC???????????????????????????????????????????
  ?????????????????
• ?????????????????

4
????

• 1956? Van Deemter ???????????,?????????????????
  ???
• ????????????,??????H?????????????????????????????
  ?????,??????????????????????????????,?????????????
  ??????

5
van Deemter??

• dp ?????????
• ????????
• Dm????????????(cm2/s)
• ????,??????
• ???????????
• q???????????,???? q?2/3
• df??????
• Ds??????????????

6
?????????
7
??????
van Deemter ???????
???????????
8
?????????
?? 2.1100mm??? 1.7µm?????? ???15,000psi ?????20
0,000????????
??????? ????????
9
UPLC?????

• ???????(???)
• ???????(???15,000psi)
• ?????????????
• ?????????????????????
• ??????,??????????
• ?????????????
• ?????????
• ??????????????

10
?????????????

• ???
• ???/??????
• ????????????
• ?UPLC????????
• ????

• ?????
• ?????????
• ????????????

• ?????
• ???XYZZ??
• ??????
• ?????
• ????/????
• ???????

• ??????
• ????
• ????
• ?????
• ????
• ?????
• UPLC?????

11
UPLC ??????!
UPLC
HPLC
0.24
??????5 5µm??? ??????6.00??
0.20
0.16

2. toluene - 1.034
3. propylbenzene - 1.742
4. butylbenzene - 2.413
0.12
AU
1. Thiourea - 0.430
0.08
5. hexylbenzene - 5.058
0.04
0.00
0.00
0.50
1.00
1.50
2.00
2.50
3.00
3.50
4.00
4.50
5.00
5.50
6.00
UPLC
??????5 1.7µm??? ???????0.60??
????????
0.20
AU
0.10
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0.40
0.45
0.50
0.55
0.60
0.00
Minutes
12
?????UPLC??????1.7?(170)!
??L/dp?UPLC????????(300)!
????? ?????
??1.7µm???????????
13
Ultra Performance LC ?????????????
60 ?? 30 ??? 70 ?????
0.050
0.040
0.030
AU
0.020
0.010
0.000
0.00
2.00
4.00
6.00
8.00
10.00
12.00
15.00
Minutes
0.050
0.040
0.030
AU
0.020
0.010
0.000
0.00
1.00
2.00
3.00
4.00
5.00
6.00
7.00
Minutes
???????????????????
14
Ultra Performance LC ?????????????
?????????????
HPLC 3.5µm
AU
UPLC 1.7µm
AU
?????????????????????????
15
Ultra Performance LC ?HPLC??????
????? N 34
16
Ultra Performance LC ?HPLC??????
???????? ?10?90???1????1000??? ??55ºC ????
8500psi ??1.3ml/min.
ACQUITY UPLC C18 ??? 2.130mm 1.7µm ??????? ??
?1000???
17
Ultra Performance LC ???MS???? - ???ID
HPLC
UPLC
18
????/?????????
19
ESI??
20
ESI?????
21
APCI??
22
???????QTOF micro

• ?????????(pg-fg?)???????????????????????,?????,???
  ?,???????????????????????????????

23
????????-??????-TOF????
24
Q-TOF MS????
25
(No Transcript)
26
(No Transcript)
27
(No Transcript)
28

• The End
• Thank you