Digital Pathology: Key Technology for Pathology Research Core Laboratories

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Digital Pathology Key Technology for Pathology
Research Core Laboratories

• Sarah Dry, MD
• Director, Translational Pathology Core Laboratory
• UCLA Department of Pathology

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Research Cores

• First funded by NCI in 1998 and 1999
• Increasing the availability of core resources
  is expected to improve the ability of cancer
  investigators to conduct research and to thereby
  facilitate scientific progress.
• Provide high-quality, cost efficient
  technologies, research materials, dedicated
  personnel and expertise
• Not affordable or practical for individual
  research labs (routine histology equipment and
  personnel set up costs 100K)

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gt250 researchers gt150 publications 9 staff (3
pathologists)
Tissue Procurement
IHC and ISH
Veterinary Pathology
TPCL
Digital Pathology
Path Consultation Services
Laser Capture Microdissection
Histology
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TPCL Digital Pathology

• Aperio XT
• Brightfield only
• Ariol SL-50
• Brightfield and fluorescence

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TPCL Digital Pathology

• Standard scanning turnaround 24 hours
• Images on CD or external hard drive
• Scans temporarily stored on our server
• Analysis
• Free initial consultation
• Analysis instruction provided
• After hours and weekend analysis possible
• Core also can perform

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13,600 slides scanned, 16 mo
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Research and Digital Pathology

• Archiving
• Automated analysis
• TPCL staff gt1,000 hours
• Publishing and presentations
• Internet viewing/conferencing

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Types of analysis in research

• Object counting (Brightfield)
• Nuclear IHC staining (Ki-67, ER, etc)
• Nuclear hematoxylin staining (cellularity,
  necrosis)
• Object shape (Brightfield)
• Area measurement (Fluorescence, Brightfield)
• Cytoplasmic IHC staining
• Vascularity / wall thickness
• Trichrome studies (fibrosis)
• Co-expression studies (Fluorescence)

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Object counting
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Object shape
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Area measurement
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Area measurement
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Quantitation of fibrosis
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Why fluorescence??
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Fluorescence multiple markers
Green Na/K ATPase Blue DAPI Red Na phosphate
co-transporter (NaPi-2)
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Fluorescence - Co-localization
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Autofluorescence
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Autofluorescence
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TPCL Digital Pathology
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Unexpected challenges

• Research slides NOT uniform
• Plastic slides
• Thick sections, especially bone
• No cover slips
• Underappreciated fluorescence demand
• Analysis
• Each project unique, cannot standardize protocols
• PIs limited understanding of automated analysis
• Variation among IHC stains from same lab (by
  hand)

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What next?

• Growth in fluorescence use (TMA)
• Increased researcher-run analysis
• Link WSDI to biorepository database
• Expanded analysis options
• Consider new scanning systems

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Making DP a success in your Core

• Identify researchers needs
• Know your limitations and capabilities
• Identify a handful of advocates
• Exploit chances to expose the technology
• Offer risk-free trials
• Convert pathology colleagues
• Monitor new advances in DP

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TPCL staff
Carmen Tosity
Garrett Gerney
Ping Fu
Nora Rozengurt
Clara Magyar, Ph.D. Lab Manager
Ngan Doan
Delia Adefuin
Missing Jonathan Said, MD
http//www.pathology.ucla.edu/tpcl/pages/
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