Microscope parts

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Microscope parts
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Microbiological medium
Culture transfer tools
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Aseptic Technique http//faculty.clintoncc.suny.ed
u/faculty/Michael.Gregory/files/Bio20102/Bio2010
220Laboratory/Prokaryotes/Procaryotes.htm
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Growth in Broth
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Streak Plate

• Procedure
•   1.  Flame the loop and wire and streak a
  loopful of broth as at A in the diagram.   2. 
  Reflame the loop and cool it.   3.  Streak as at
  B to spread the original inoculum over more of
  the agar.   4.  Reflame the loop and cool it.  
  5.  Streak as at C.   6.  Reflame the loop and
  cool it.   7.  Streak as at D.   8.  Label the
  plate and incubate it inverted.

 
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Colony Morphology
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Smear preparation
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coccus
spiral
rod
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Coccus arrangements
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Negative stain prep
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rod
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Gram stain procedure
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• Gram stain

Gram positive
Gram negative
Mixed
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Gram vs Gram- cell walls
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(No Transcript)
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Spore stain
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Capsule Stain
capsule
cell
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Flagella stain
amphitrichous
monoitrichous
lophphotrichous
peritrichous
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(No Transcript)
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M. tuberculosis colonies on Lowenstein-Jensen
medium
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Acid Fast Stain
Acid Fast
Non Acid Fast
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Lowenstein Jensen medium