PowerPoint Presentation - A UserGuide for the INOVA 300

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Varian UnityInova 300 NMR User Guide
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A UserGuide for the INOVA 300
Temple Chemistry Department NMR Facility July 2009
Rev 2.2 10 July 2009
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Some Computer Information
This password is also to be used if the screen
saver has locked you out at the console. In this
manual, the LMB instruction means click the
leftmost of the three mouse buttons. RMB means
click the rightmost mouse button. ltrtngt means
press the return or enter key. Dont type the
letters or the brackets or the quotes.
Finding the Inova 300 over the Network..   Networ
k Name of the computer nmr300.chem.temple.edu   lo
gin ID temple   Password for temple chem300   data
pathway /home/temple/vnmrsys/data/
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Getting Started

• There will be a chloroform standard in the magnet
  when you start
• Put the mouse in the command window, type e
  ltrtngt .ltrtngt means carriage return or enter.
  Dont type the quotation marks)
• Remove the existing CDCl3 sample, and exchange
  your sample for the chloroform in the spinner
  collar.
• Position your tube using the depth gauge marked
  300, wipe with kimwipe
• Put your sample into the top of the magnet bore
• With the mouse in the command window, type i
  ltrtngt Your sample will lower into the magnet

shimnow

If the blue prompter in the command line is not
solid, click into the command line with the LMB
(left mouse button) Next, on the command line,
type shimnowltrtngt
   
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Now you select the automated protocol to be used
Mouse to the side bar buttons and left Click
(LMB) on the button labeled CustomQ
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Next Make the Choices for your experiment
First select the deuterated solvent for locking.
This button lets you drag down to the solvent
choice.
Next, mouse to the center block of buttons. These
are labeled e.g. H1only LMB click the proper
choice
Some of the other buttons give choices for
linked, successive experiments, such as proton
followed by COSY. Experiments are done in the
order selected. Be sure to note the time
requirements, before starting.
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Now the File-naming Dialog Box Appears
Now many actions flash across the screen. When it
stops, you will see a dialog box. Typically you
will leave the default choices about locking,
plotting and shimming engaged, as shown here.
LMB click in the Save As field, and at the
insertion point, type your user name (which you
will be given, use this consistently) then type
/ and then an appropriate filename. Make this
systematic, so it will make sense later. Your
notebook code is a useful convention. In this
field, do not use spaces, asterisks, tildes, or
other slashes (these are reserved for directory
pointing) Type ltrtngt when you are finished. You
may enter some descriptive text into the lower
box. Then LMB OK, then Exit
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Now Another Dialog box Appears
In this Example We Had Selected the H-1 Only
Button from CustomQ   We can choose some
parameter options here, by LMB clicking on the
indicated values. Here we have chosen Spectral
Width from -0.5 to 9.5 ppm, and 32 scans, along
with a 2-second delay between repetitions. Your
choice will become shaded as you select it.   LMB
OK, Exit
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An Option--Change the Set Up Parameters
You may change some of the conditions before
starting the experiment. In the sidebar, click
(LMB) the AcqObs tab. In the boxes on this
screen you might alter the delay time or the
number of pulses. Then, LMB CustomQ
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You are Almost Ready to Start! Reclick into the
CustomQ Menu
You need to do this action again, to get to the
Start ACQ button LMB click on the Start ACQ
button.
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Now the Automatic Locking and Shimming Takes over
You will see the banner that tells you what is
happening. Your role is to watch!   The message
window will display some RMS err output, during
this process. This is not an error, but normal.
The gradient shimming will finish when the
agreement is gt1
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After the Shimming finishes, the Scanning starts
The Acquisition Status window tells you what is
happening, and how far has experiment proceeded
(CT, completed transients) This is also the
window that tells you if e.g. the autolock has
failed. Information about the spinner status
etc. also is reported
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If you need to Halt an experiment
For example, if the lock or spinning
Fails.    After the Acquisition Status window
shows that the job is idle, you can eject, change
CustomQ choices, put a new sample in etc.
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When the experiment is Finished
When the job is finished, it will be
automatically processed (FT, phased etc.), then
plotted on the laser printer.     You can pick up
the hard copy, and reverse the steps on page 1,
to retrieve your sample and replace the CDCl3
standard.   Your Spectrum can be FTP transferred
over the network for NUTs processing. You should
navigate to nmr300.chem.temple.edu
/home/temple/vnmrsys/data/(your name) /(todays
experiment)
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Last Step
Remove your sample by reversing the steps on page
2.   Replace with the standard sample, and
insert.   Sign the logbook to record your use,
files created, and any comment on instrument
problems.