AGARIC DIVERSITY IN THE GREAT SMOKY MOUNTAINS NATIONAL PARK - PowerPoint PPT Presentation

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AGARIC DIVERSITY IN THE GREAT SMOKY MOUNTAINS NATIONAL PARK

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Three years of collections by experts in various groups of agaric fungi ... Goal to link to photos and records in next three years. SPONSORS. Karen Hughes ... – PowerPoint PPT presentation

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Title: AGARIC DIVERSITY IN THE GREAT SMOKY MOUNTAINS NATIONAL PARK


1
AGARIC DIVERSITY IN THE GREAT SMOKY MOUNTAINS
NATIONAL PARK
  • PART OF THE GSMNP ALL TAXA BIODIVERSITY INVENTORY
    (ATBI)

2
  • PROJECT GOALS
  • Three years of collections by experts in various
    groups of agaric fungi
  • Databasing of current and past collections
  • Bar coding of species using ribosomal ITS region
  • Species pages available on web

3
TENN
  • Tennessee herbarium web site lists 1500 species
    from the GSMNP
  • Goal to link to photos and records in next
    three years

4
PARTICIPANTS Aime, Cathy Rusts and
Crepidotus Ammirati, Joe - Cortinarius Baroni,
Tim Entoloma Buyck, Bart Russula / Lactarius
Caranza, Julietta - Trametes Cifuentes, Joaquin
- General Halling, Roy Gymnopus and
others Horak, Egon - General Kerrigan, Rick -
Agaricus Lodge, Jean - Hygrophoraceae Mata, Juan
Luis - Gymnopus Matheny, Brandon -
Inocybe McCleneghan, Coleman - Pholiota Methven,
Andrew - Lactarius Ovrebo, Clark -
Tricholoma Tulloss, Rod -Amanita Verbaken,
Anameka Russula /Lactarius
COVERAGE
SPONSORS Karen Hughes Ron Petersen Ed
Lickey Gymnopus 450 sequences Collybia Omphalot
us Artomyces (Clavicorona) Lentinellus Lentinula
Xerula Ramaria
Andrew Miller - Pyrenomycetes
5
(No Transcript)
6
  • TWO PROBLEMS FOR BARCODING USING THE ITS REGION
  • PROBLEM 1
  • INABILITY TO DISTINGUISH SOME APPARENT
    MORPHOSPECIES (GYMNOPUS SECT LEVIPEDES)
  • But does distinguish a number of Gymnopus
    collections previously grouped as the same
    morphospecies (cryptic species)

7
  • TWO PROBLEMS FOR BARCODING USING THE ITS REGION
  • PROBLEM 2
  • THE ITS REGION IN COLLECTIONS FROM THE GSMNP HAS
    RELATIVELY HIGH LEVELS OF HETEROZYGOSITY FOR
    INDELS.
  • Requires cloning with its attendant problems

8
Cortinarius sp. 31.3
Lactarius sp. 25.0
Hygrophorus 57.7
Saprobes 28.5
9
  • PROPORTION OF COLLECTIONS FROM THE GSMNP THAT
    WERE ALREADY REPRESENTED IN GENBANK
  • 98 CONGRUENCE WAS ARBITRARILY CONSIDERED TO BE
    CONSPECIFIC
  • 159/533 MATCHED SPECIES IN GENBANK
  • 23/533 MATCHED UNNAMED ENVIRONMENTAL SAMPLES
  • 351/533 HAD NO MATCH IN GENBANK
  • FOR AGARICS, SEQUENCE COVERAGE IS STILL
    SIGNIFICANTLY INCOMPLETE

10
GSMNP BAR CODING PROJECT?
  • Home of an ATBI
  • Center of Biodiversity
  • Highly varied terrain /ecosystems
  • Nice collecting spot
  • Good field station
  • On the ground help
  • Need I say more?
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