Considerations for Flow Measurement on the Mouse Femoral Artery

1
Considerations for Flow Measurement on the Mouse
Femoral Artery

• Location of Measurement with the Flowprobe
• Vessel Diameter
• Maintaining Body Temperature
• Experimental Set-up

Photos and data courtesy of Michael F.
Callahan Depts. of Orthopaedic Surgery,
Physiology Pharmacology, Wake Forest
University Health Sciences Winston-Salem, North
Carolina
All information in this file is privileged
information and may not be used without
permission of the author.
2
Branches of Femoral Artery Before and After
Dissection
Epigastric Nerve, Artery and Vein
Approximate Location of Knee
Femoral Nerve, Artery and Vein exiting from
abdominal wall
Femoral Nerve, Superficial Femoral Artery and Vein
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Femoral artery proximal to the epigastric a.,
isolated from the femoral vein, measures 400 µm
diameter with a micrometer. Flow measurements
can be made in this location easily.
4
Femoral Blood Flow in Anesthetized Mouse Recorded
with Transonic Systems Flowmeter Nanoprobe
Data Courtesy of Michael F. Callahan, Wake Forest
Health Sciences, Winston-Salem, NC
MFCallahan, 3/03
5
Effect of Core Temperature on Femoral Blood Flow
(22g CD-1, 3-16-03)
Temperature has a profound effect on femoral
blood flow and must be monitored.
Data Courtesy of Michael F. Callahan, Wake Forest
Health Sciences, Winston-Salem, NC
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Flow measurements on the femoral artery distal to
the epigastric a.
Isolation of the artery from the vein in this
position is very challenging. There is less
fascia and connective tissue around the sheath of
the artery and vein to manipulate the vessel
free. The nerve runs immediately adjacent and
is very easy to tear. Once isolated, avoid
pulling up on the artery which would cause
vascular spasm or putting pressure on the vein to
cause occlusion.
Superficial femoral a.
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The superficial femoral artery, distal to the
epigastric a., is isolated from the femoral vein
and measures 200 µm diameter on the scale.
8
Transonic 0.5PSB flowprobe on the superficial
femoral artery distal to the epigastric a.
9
Sample flow trace from lower on femoral artery
near the knee. Since the vessel diameter fills
lt 60 of the probe lumen, probe position must
remain fixed for a stable measurement.
32g male CD-1 with 0.5PSB probe on superficial
femoral artery Temperature 33o C.
Michael Callahan, Wake Forest Univ. Health
Sciences, 2/5/2004
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Experimental Setup for Femoral Artery Blood Flow
Study
Close up of the Transonic nanoprobe handle
clamped in a micromanipulator with two axis
adjustment to fine tune and stabilize position of
probe for measurement.
11
Experimental Setup for Femoral Artery Blood Flow
Study
TransonicSystems 400-Series Flowmeter Console
Probe holder with magnetic base, adjustable arm,
and micro-positioning adjustment
Heating pad lamp used with gauze cover to
maintain mouse core temperature. Temp. is
monitored with an anal probe.
12
Simultaneous Femoral Artery Blood Flow Pressure
Recording
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Flow recorded from the superficial femoral a.
with occlusion Pressure recorded from the
contra lateral femoral a.
14
Transonic Systems, Inc. gratefully acknowledges
the investigators for the presentation of this
data on the Transonic website. All information
in this file is privileged information and may
not be used without permission of the authors.

Photos and data courtesy of Michael F.
Callahan, PhD Thomas L. Smith, PhD Depts. of
Orthopaedic Surgery, Physiology
Pharmacology, Wake Forest University Health
Sciences Winston-Salem, North Carolina Edited by
Margo Sosa, Sr. Product Manager Transonic
Systems, Inc. www.transonic.com December 2004