Metabolomics: What do we mean by it and what can it tell us

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Metabolomics What do we mean by it and what
can it tell us?
Chaired by Anne-Marie DELORT, Evelyne FORANO
Annick MOING
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From metabolism to metabolomics
METABOLISM of a specific compound
ECOSYSTEMS ORGANISMS TISSUES ORGANS
INDICATORS BIOMARKERS diagnostic,
functional genomic
STUDY OFMECHANISMS Biochemistry, molecular
physiology, flux
METABOLOMICS
CELLS COMPARTMENTS
LARGE EXTEND OF METABOLITES AND PATHWAYS
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Annick MOING "Plant Metabolomics how and what
for ? "
6 steps 1- sampling (storage) 2- metabolite
extraction (standardisation, reproducibility) 3-
biochemical analysis (GC-MS, LC-MS, NMR) 4- data
pre-processing (base line correction.) 5- data
visualisation and mining (PCA, data bases) 6-
integration of data (metabolic pathways,
genome..)
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Examples related to gut microbiology

• Effect of Xenobiotics or environment factors on
  metabolism
• Uncovering metabolic changes using a combined
  LC-MS/NMR approach Ochratoxins effect in sheep.
  ( D. MORGAVI )
• Central metabolism (sugars, amino acids)
• Carbohydrate metabolism in Fibrobacter
  succinogenes (E. FORANO)
• Metabolism of Xenobiotics (drugs, micronutrients,
  food additives)
• Metabolism of the food associated carcinogen PhIP
  by human intestinal microbiota (L. VANHAECKE)

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Concluding remarks
Analogy with transcriptomics and proteomics
(masses of information to treat).