IMViC

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IMViC

• Indole (3 tests)
• Methyl Red
• Voges-Proskauer
• Citrate
• Experiment 25 D

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IMViC

• EnterobacteriaceaFound in our intestinal tract,
  Gram (-), spore (-), cocci.
• Some are pathogens, occasional pathogens and
  normal intestinal flora.
• E. coli Enterobacter are normal flora when in
  intestinal tract, pathogenic to other parts of
  our bodies.
• This differentiates Enterobacteriaceae into
  lactose fermenters and nonfermenters.

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Citrate

• Objectivedetermine the ability of your organism
  to ferment citrate as its only source of carbon.
• Reaction depends on organism having a membrane
  proteincitrate permease, this is a transport
  protein facilitating transport of citrate into
  the cell.
• Inside cell
• Citrategtcitrasegtoxalacetic acetic acids
• Oxalacetic acidgt pyruvic acid CO2

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Citrate

• The waste product carbon dioxide reacts with
  other products in the media
• Carbon dioxide sodium water Sodium
  Carbonate
• Sodium Carbonate is alkaline pH rises
• pH indicator Bromthymol Blue
• pH neutralgreen (-)
• pH 7.6 (alkaline)blue ()

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Citrate

• Inoculate Simmons Citrate medium
• Streak and stab the tube 37C
• Read results
• Thursday Tuesday
• Green (-)
• Blue () -

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Citrate

• Objective
• fermentation of citrate
• Citrate permease transports
• citrate inside cell
• waste product CO2
• Citrategtcitrasegtoxalacetic acetic acids
• Oxalacetic acid pyruvic acid CO2
• Carbon dioxide sodium water Sodium
  Carbonate
• pH 7.6 (alkaline) blue ()

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