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Title:

Proteomics

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Compare all proteins in diseased ... Further References. Ebook: Introduction to Proteomics by Dan Liebler: ... Ebook: Genomics and Proteomics by Suhai Sandor: ... – PowerPoint PPT presentation

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Title: Proteomics


1
Proteomics
  • Laurie.Earls_at_Vanderbilt.edu

2
Learning Objectives
  • Define Proteomics
  • What sorts of questions does proteomics answer?
  • Some examples of proteomic techniques

3
Perspective on Proteomics
  • Genome
  • Genomics
  • Examples
  • Microarray
  • Proteome
  • Proteomics
  • Examples

4
More is better
  • There is only one genome
  • There are multiple proteomes

5
The old way of studying cell function
Early 1980s-Ras discovered
20 years later…
6
The Proteomics Way
  • Compare all proteins in diseased vs. normal
    tissue
  • Determine all proteins which bind to a certain
    target
  • Determine all changes in proteins (ex
    phosphorylation) that occur in a particular state

7
More fun vocabulary
  • Bioinformatics http//us.expasy.org/prosite/
  • Lipidomics

8
What aspects of proteins can be studied in
massive scale?
9
Proteomics is a beginning not an end
Complementary methods
  • Binding partners Co-IP, GST-Pulldowns
  • Modification state Phospho antibodies
  • Localization IHC
  • Expression levels Northern, Southern, RT-PCR,
    western
  • Dont forget function!!!!!!!!!

10
Mass Spectrometry
Resolved ions
ions
Source
Mass analyzer
detector
Ex TOF quadrupole Ion trap
Ex MALDI ESI
  • For Proteomics, Need
  • Sensitivity(can read peptides in femtomole
    quantities)
  • Resolution(can distinguish w/similar masses(ie
    the difference in size of one H atom))
  • Accuracy(Masses obtained are very close to
    actuality)

11
How to interpret a Mass spectra
http//www.ionsource.com/tutorial/spectut/spec1.ht
m
12
Further References
Ebook Introduction to Proteomics by Dan
Liebler http//www.netlibrary.com/ebook_info.as
p?product_id71291
Ebook Genomics and Proteomics by Suhai
Sandor http//www.netlibrary.com/ebook_info.asp?p
roduct_id67124
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