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Chapter 5 Nutrition, Culture, and Metabolism of Microorganisms

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a. Krebs cycle. b. electron transport chain. c. in yeast, conversion of pyruvic acid ... Question: What is the purpose of the Krebs cycle? a. to make ATP ... – PowerPoint PPT presentation

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Title: Chapter 5 Nutrition, Culture, and Metabolism of Microorganisms


1
Chapter 5 Nutrition, Culture, and Metabolism of
Microorganisms
Oxidation-Reduction Reactions
Nutrition and Culture
Energetics and Enzymes
Essentials of Catabolism
Essentials of Anabolism
100
100
100
100
100
200
200
200
200
200
300
300
300
300
300
400
400
400
400
400
500
500
500
500
500
FINAL ROUND
2
Topic 1 Nutrition and Culture 100 Question
  • Question Siderophores help transfer which of the
    following macronutrients across the cell
    membrane?
  • a. nitrogen
  • b. phosphorous
  • c. iron
  • d. potassium

ANSWER
BACK TO GAME
3
Topic 1 Nutrition and Culture 100 Answer
  • Question Siderophores help transfer which of the
    following macronutrients across the cell
    membrane?
  • a. nitrogen
  • b. phosphorous
  • c. iron
  • d. potassium

BACK TO GAME
4
Topic 1 Nutrition and Culture 200 Question
  • Question Which of the following macronutrients
    makes up both amino acids and nucleotides?
  • a. nitrogen
  • b. phosphorous
  • c. iron
  • d. potassium

ANSWER
BACK TO GAME
5
Topic 1 Nutrition and Culture 200 Answer
  • Question Which of the following macronutrients
    makes up both amino acids and nucleotides?
  • a. nitrogen
  • b. phosphorous
  • c. iron
  • d. potassium

BACK TO GAME
6
Topic 1 Nutrition and Culture 300 Question
  • Question Which of the following is considered a
    growth factor?
  • a. iron
  • b. vitamins
  • c. hydroxamic Acid
  • d. potassium

ANSWER
BACK TO GAME
7
Topic 1 Nutrition and Culture 300 Answer
  • Question Which of the following is considered a
    growth factor?
  • a. iron
  • b. vitamins
  • c. hydroxamic Acid
  • d. potassium

BACK TO GAME
8
Topic 1 Nutrition and Culture 400 Question
  • Question In which type of media would an
    investigator add specific factors or nutrients
    that would allow certain organisms to grow that
    would not normally grow?
  • a. chemically defined
  • b. dIfferential
  • c. selective
  • d. enrichment

ANSWER
BACK TO GAME
9
Topic 1 Nutrition and Culture 400 Answer
  • Question In which type of media would an
    investigator add specific factors or nutrients
    that would allow certain organisms to grow that
    would not normally grow?
  • a. chemically defined
  • b. differential
  • c. selective
  • d. enrichment

BACK TO GAME
10
Topic 1 Nutrition and Culture 500 Question
  • Question Which type of media allows the bacteria
    to become visible and form colonies?
  • a. liquid culture
  • b. solid culture
  • c. chemically defined media
  • d. chemically complex media

ANSWER
BACK TO GAME
11
Topic 1 Nutrition and Culture 500 Answer
  • Question Which type of media allows the bacteria
    to become visible and form colonies?
  • a. liquid culture
  • b. solid culture
  • c. chemically defined media
  • d. chemically complex media

BACK TO GAME
12
Topic 2 Energetics and Enzymes100 Question
  • Question If ?G0' lt 0, then _______________.
  • a. the reaction is exergonic and can occur
    spontaneously
  • b. the reaction is endergonic and can occur
    spontaneously
  • c. the reaction is exergonic and cannot occur
    spontaneously
  • d. the reaction is endergonic and cannot occur
    spontaneously

ANSWER
BACK TO GAME
13
Topic 2 Energetics and Enzymes100 Answer
  • Question If ?G0' lt 0, then _______________.
  • a. the reaction is exergonic and can occur
    spontaneously
  • b. the reaction is endergonic and can occur
    spontaneously
  • c. the reaction is exergonic and cannot occur
    spontaneously
  • d. the reaction is endergonic and cannot occur
    spontaneously

BACK TO GAME
14
Topic 2 Energetics and Enzymes200 Question
  • Question Which of the following are
    characteristics of enzymes?
  • a. they are consumed by the enzyme-
    mediated reaction
  • b. they are not altered by the enzyme-
    mediated reaction
  • c. they raise activation energy
  • d. all of the above

ANSWER
BACK TO GAME
15
Topic 2 Energetics and Enzymes200 Answer
  • Question Which of the following are
    characteristics of enzymes?
  • a. they are consumed by the enzyme-
    mediated reaction
  • b. they are not altered by the enzyme-
    mediated reaction
  • c. they raise activation energy
  • d. all of the above

BACK TO GAME
16
Topic 2 Energetics and Enzymes300 Question
  • Question Which of the following terms relate to
    enzymes?
  • a. proteins
  • b. active site
  • c. catalysts
  • d. all of the above

ANSWER
BACK TO GAME
17
Topic 2 Energetics and Enzymes300 Answer
  • Question Which of the following terms relate to
    enzymes?
  • a. proteins
  • b. active site
  • c. catalysts
  • d. all of the above

BACK TO GAME
18
Topic 2 Energetics and Enzymes400 Question
  • Question How do you calculate the ?G0' of a
    reaction?
  • a. add the Gf0 of all of the products and then
    subtract the Gf0 of all of the reactants
  • b. add the Gf0 of all of the reactants and then
    add the Gf0 of all of the products
  • c. subtract the Gf0 of any products and add
    the Gf0 of the reactants
  • d. subtract the Gf0 of any reactants and then
    add the Gf0 of the products

ANSWER
BACK TO GAME
19
Topic 2 Energetics and Enzymes400 Answer
  • Question How do you calculate the ?G0' of a
    reaction?
  • a. add the Gf0 of all of the products and then
    subtract the Gf0 of all of the reactants
  • b. add the Gf0 of all of the reactants and then
    add the Gf0 of all of the products
  • c. subtract the Gf0 of any products and add
    the Gf0 of the reactants
  • d. subtract the Gf0 of any reactants and then
    add the Gf0 of the products

BACK TO GAME
20
Topic 2 Energetics and Enzymes500 Question
  • Question Which of the following molecules do
    enzymes associate with in catalyzing a reaction?
  • a. prosthetic groups
  • b. coenzymes
  • c. both prosthetic groups and coenzymes
  • d. neither prosthetic groups nor coenzymes

ANSWER
BACK TO GAME
21
Topic 2 Energetics and Enzymes500 Answer
  • Question Which of the following molecules do
    enzymes associate with in catalyzing a reaction?
  • a. prosthetic groups
  • b. coenzymes
  • c. both prosthetic groups and coenzymes
  • d. neither prosthetic groups nor coenzymes

BACK TO GAME
22
Topic 3 Oxidation-Reduction Reactions 100
Question
  • Question If organism A used H2S as an electron
    donor and Fe3 as an electron acceptor (at pH of
    7) and organism B used methanol as an electron
    donor and O2 as an electron acceptor, which
    organism can probably produce most energy.
  • a. organism A
  • b. organism B
  • c. both produce probably the same amount
    of energy

ANSWER
BACK TO GAME
23
Topic 3 Oxidation-Reduction Reactions 100
Answer
  • Question If organism A used H2S as an electron
    donor and Fe3 as an electron acceptor (at pH of
    7) and organism B used methanol as an electron
    donor and O2 as an electron acceptor, which
    organism can probably produce most energy.
  • a. organism A
  • b. organism B
  • c. both produce probably the same amount
    of energy

BACK TO GAME
24
Topic 3 Oxidation-Reduction Reactions 200
Question
  • Question Which of the following statements
    concerning NADH is true compared to other
    molecules in the electron transport chain?
  • a. it has the most negative E0 and the greatest
    capacity to accept electrons
  • b. it has the most negative E0 and the greatest
    capacity to donate electrons
  • c. it has the most positive E0 and the greatest
    capacity to accept electrons
  • d. it has the most positive E0 and the greatest
    capacity to donate electrons

ANSWER
BACK TO GAME
25
Topic 3 Oxidation-Reduction Reactions 200
Answer
Question Which of the following statements
concerning NADH is true compared to other
molecules in the electron transport chain? a. it
has the most negative E0 and the greatest
capacity to accept electrons b. it has the most
negative E0 and the greatest capacity to donate
electrons c. it has the most positive E0 and the
greatest capacity to accept electrons d. it has
the most positive E0 and the greatest capacity
to donate electrons
BACK TO GAME
26
Topic 3 Oxidation-Reduction Reactions 300
Question
  • Question Which of these groups are carried by
    NADH and FADH2 and will ultimately lead to the
    production of ATP in the electron transport
    chain?
  • a. electrons
  • b. hydrogen ions
  • c. acetyl CoA
  • d. phosphorous
  • e. a and b

ANSWER
BACK TO GAME
27
Topic 3 Oxidation-Reduction Reactions 300
Answer
  • Question Which of these groups are carried by
    NADH and FADH2 and will ultimately lead to the
    production of ATP in the electron transport
    chain?
  • a. electrons
  • b. hydrogen ions
  • c. acetyl CoA
  • d. phosphorous
  • e. a and b

BACK TO GAME
28
Topic 3 Oxidation-Reduction Reactions 400
Question
  • Question Which of the following molecules could
    serve as an electron donor in the electron
    transport chain?
  • a. FAD
  • b. NADH
  • c. NAD
  • d. NADPH

ANSWER
BACK TO GAME
29
Topic 3 Oxidation-Reduction Reactions 400
Answer
  • Question Which of the following molecules could
    serve as an electron donor in the electron
    transport chain?
  • a. FAD
  • b. NADH
  • c. NAD
  • d. NADPH

BACK TO GAME
30
Topic 3 Oxidation-Reduction Reactions 500
Question
  • Question During which of the following processes
    does NAD get regenerated so that it can be used
    again in glycolysis?
  • a. Krebs cycle
  • b. electron transport chain
  • c. in yeast, conversion of pyruvic acid to
    ethanol and carbon dioxide
  • d. a and c
  • e. b and c

ANSWER
BACK TO GAME
31
Topic 3 Oxidation-Reduction Reactions 500
Answer
  • Question During which of the following processes
    does NAD get regenerated so that it can be used
    again in glycolysis?
  • a. Krebs cycle
  • b. electron transport chain
  • c. in yeast, conversion of pyruvic acid to
    ethanol and carbon dioxide
  • d. a and c
  • e. b and c

BACK TO GAME
32
Topic 4 Prokaryotic Structures 100 Question
  • Question Which of the following reactions in
    glycolysis involve substrate-level
    phosphorylation?
  • a. phosphoenolpyruvate ? pyruvate
  • b. glucose? Glucose-6-phosphate
  • c. 1,3 bisphosphoglycerate ? 3- phosphoglycerat
    e
  • d. a and b
  • e. a and c

ANSWER
BACK TO GAME
33
Topic 4 Essentials of Catabolism 100 Answer
  • Question Which of the following reactions in
    glycolysis involve substrate-level
    phosphorylation?
  • a. phosphoenolpyruvate ? pyruvate
  • b. glucose? Glucose-6-phosphate
  • c. 1,3 bisphosphoglycerate ? 3- phosphoglycerate
  • d. a and b
  • e. a and c

BACK TO GAME
34
Topic 4 Essentials of Catabolism 200 Question
  • Question What is the purpose of the Krebs cycle?
  • a. to make ATP
  • b. to completely oxidize the pyruvate that
    comes from glycolysis
  • c. to form molecules that will shuttle
    electrons to the electron transport chain
  • d. to make some intermediates that can be used
    during biosynthesic reactions
  • e. all of the above

ANSWER
BACK TO GAME
35
Topic 4 Essentials of Catabolism 200 Answer
  • Question What is the purpose of the Krebs cycle?
  • a. to make ATP
  • b. to completely oxidize the pyruvate that
    comes from glycolysis
  • c. to form molecules that will shuttle
    electrons to the electron transport chain
  • d. to make some intermediates that can be used
    during biosynthesic reactions
  • e. all of the above

BACK TO GAME
36
Topic 4 Essentials of Catabolism 300 Question
  • Question During which stage of cellular
    respiration are both NADH and FADH2 produced?
  • a. glycolysis
  • b. conversion of pyruvate to acetyl CoA
  • c. Krebs cycle
  • d. electron transport chain

ANSWER
BACK TO GAME
37
Topic 4 Essentials of Catabolism 300 Answer
  • Question During which stage of cellular
    respiration are both NADH and FADH2 produced?
  • a. glycolysis
  • b. conversion of pyruvate to acetyl CoA
  • c. Krebs cycle
  • d. electron transport chain

BACK TO GAME
38
Topic 4 Essentials of Catabolism 400 Question
  • Question What is the correct order of transfer
    of electrons in the electron transport chain?
  • a. oxygen, cytA, cytC, Q, FMN, NADH
  • b. FMN, NADH, Q, cytC, cytA, oxygen
  • c. NADH, FMN, Q, cytC, cytA, oxygen
  • d. cytA, oxygen, cytC, NADH, FMN, Q

ANSWER
BACK TO GAME
39
Topic 4 Essentials of Catabolism 400 Answer
  • Question What is the correct order of transfer
    of electrons in the electron transport chain?
  • a. oxygen, cytA, cytC, Q, FMN, NADH
  • b. FMN, NADH, Q, cytC, cytA, oxygen
  • c. NADH, FMN, Q, cytC, cytA, oxygen
  • d. cytA, oxygen, cytC, NADH, FMN, Q

BACK TO GAME
40
Topic 4 Essentials of Catabolism 500 Question
  • Question Where are the different complexes of
    the electron transport chain located in the
    mitochondria?
  • a. outer membrane
  • b. inner mitochondrial membrane
  • c. inner membrane space
  • d. matrix

ANSWER
BACK TO GAME
41
Topic 4 Essentials of Catabolism 500 Answer
  • Question Where are the different complexes of
    the electron transport chain located in the
    mitochondria?
  • a. outer membrane
  • b. inner mitochondrial membrane
  • c. inner membrane space
  • d. matrix

BACK TO GAME
42
Topic 5 Essentials of Anabolism100 Question
  • Question In prokaryotics, polysaccharides are
    synthesized from either _______________.
  • a. UDPG or NADPH
  • b. NAM or ADPG
  • c. UDPG or ADPG
  • d. UDPG or NAG

ANSWER
BACK TO GAME
43
Topic 5 Essentials of Anabolism 100 Answer
  • Question In prokaryotics, polysaccharides are
    synthesized from either _______________.
  • a. UDPG or NADPH
  • b. NAM or ADPG
  • c. UDPG or ADPG
  • d. UDPG or NAG

BACK TO GAME
44
Topic 5 Essentials of Anabolism 200 Question
  • Question The process of synthesizing glucose in
    a cell is called _______________.
  • a. anaerobic respiration
  • b. glutamine synthetase
  • c. gluconeogenesis
  • d. fermentation
  • e. anabolism

ANSWER
BACK TO GAME
45
Topic 5 Essentials of Anabolism 200 Answer
  • Question The process of synthesizing glucose in
    a cell is called _______________.
  • a. anaerobic respiration
  • b. glutamine synthetase
  • c. gluconeogenesis
  • d. fermentation
  • e. anabolism

BACK TO GAME
46
Topic 5 Essentials of Anabolism 300 Question
  • Question The enzyme ribonucleotide reductase is
    used in the synthesis of _______________.
  • a. DNA
  • b. RNA
  • c. amino acids
  • d. lipids

ANSWER
BACK TO GAME
47
Topic 5 Essentials of Anabolism 300 Answer
  • Question The enzyme ribonucleotide reductase is
    used in the synthesis of _______________.
  • a. DNA
  • b. RNA
  • c. amino acids
  • d. lipids

BACK TO GAME
48
Topic 5 Essentials of Anabolism 400 Question
  • Question Which of the following is used in the
    biosynthesis of amino acids and is produced in
    the Krebs cycle?
  • a. glutamate
  • b. glutamine
  • c. a-ketoglutarate
  • d. ammonia

ANSWER
BACK TO GAME
49
Topic 5 Essentials of Anabolism 400 Answer
  • Question Which of the following is used in the
    biosynthesis of amino acids and is produced in
    the Krebs cycle?
  • a. glutamate
  • b. glutamine
  • c. a-ketoglutarate
  • d. ammonia

BACK TO GAME
50
Topic 5 Essentials of Anabolism 500 Question
  • Question Inosinic acid is the precursor of
    _______________.
  • a. adenine and guanine
  • b. adenine and cytosine
  • c. thymine and cytosine
  • d. guanine and thymine

ANSWER
BACK TO GAME
51
Topic 5 Essentials of Anabolism 500 Answer
  • Question Inosinic acid is the precursor of
    _______________.
  • a. adenine and guanine
  • b. adenine and cytosine
  • c. thymine and cytosine
  • d. guanine and thymine

BACK TO GAME
52
FINAL ROUND Question
  • Question If something was not working properly
    in the cell and pyruvate could not be converted
    into acetyl CoA, then what would be the total
    production of ATP at the end of all the stages in
    cellular respiration that could occur?
  • a. 2
  • b. 8
  • c. 14
  • d. 36

ANSWER
BACK TO GAME
53
FINAL ROUND Answer
  • Question If something was not working properly
    in the cell and pyruvate could not be converted
    into acetyl CoA, then what would be the total
    production of ATP at the end of all the stages in
    cellular respiration that could occur?
  • a. 2
  • b. 8
  • c. 14
  • d. 36

BACK TO GAME
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