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Nucleic Acids 101

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Nucleic Acids 101. Last week's grand challenge: Reading and interpreting. genetic information ... A chromosome has a single DNA molecule running the length of ... – PowerPoint PPT presentation

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Title: Nucleic Acids 101


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Nucleic Acids 101
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Last weeks grand challenge Reading and
interpreting genetic information
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DNA Repair DNA polymerase 10-7 Mismatch repair
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Genomics
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Restriction Nucleases
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Ligation
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Grand challenge Every disease requires a
tremendous amount of interdisciplinary effort to
understand and fight it. Can this be streamlined?
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The frequency of crossing-over is
proportional to the physical distance
between them. Find DNA markers close to and
flanking the gene of interest
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Positional Cloning
  • A chromosome has a single DNA molecule running
    the length of the chromosome
  • Map the gene for the trait in question in that
    DNA molecule identify close flanking markers
  • Walk between the flanking markers
  • Identify the gene in question between the markers

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How do you map a gene?
  • Collect DNA samples from family members in a
    pedigree
  • Seek linkage to a polymorphic DNA marker
  • Take advantage of the Human Genome Project to
    identify the chromosomal region
  • Narrow down the location in the overlapping clones

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What are DNA markers?
  • RFLP--Restriction Fragment Length Polyhmorphism
  • SNP--Single Nucleotide Polymorphisms

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Mutation destroys a restriction site,
causing a RFLP
(This is also a SNP--Single Nucleotide
Polymorphism)
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RFLP
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STRP or VNTR
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Pedigree of Huntington Disease
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SNPs Single Nucleotide Polymorphisms
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NEXT CLASS Central Dogma
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