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The Compound Microscope

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... View (FV) is the illuminated circle that you see when looking ... Scanning Electron Microscopes (SEM) (C) Explain how to prepare a wet mount . TEM. SEM ... – PowerPoint PPT presentation

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Title: The Compound Microscope


1
The Compound Microscope
2
Parts of Compound Microscope
3
Parts / Functions
  • 1.Ocular Eyepiece structure you look through to
    examine the specimen contains a lens with a
    magnification of 10x.
  • 2.Revolving Nosepiece moves the desired
    objective (lens) into place.
  • 3.Objectives are the 3 lenses
  • low or scanning power 4x
  • medium power 10x
  • high power 40x

4
  • 4.Arm supports the ocular eyepiece, revolving
    nosepiece, objectives.
  • 5.Stage Clip holds the slide with the specimen
    in place.
  • 6.Stage supports the slide and moves up or
    down.
  • 7.Diaphragm controls the amount of light that
    passes through the specimen.

5
  • 8. Illuminator light source.
  • 9.Coarse Focus Adjustment Knob used only under
    low power it moves the stage a great distance to
    bring the specimen into focus.
  • 10.Fine Focus Adjustment Knob can be
    used under any objective moves the stage a small
    distance to fine tune the image.

6
  • 11. Base supports the microscope.
  • 12. On / Off Switch

7
Magnification
8
TOTAL MAGNIFICATION
  • Powers of the eyepiece (10X) multiplied by
    objective lenses determine total magnification.

9
Field of View
  • Field of View (FV) is the illuminated circle that
    you see when looking through the ocular eyepiece.
  • If we know the diameter of the FV then we can
    estimate the size of our microorganisms.

10
Field of View
  • With our microscopes the diameter of the FV under
    low power is 4 mm
  • FV is measured in micrometers or microns.
  • 1 mm 1000 microns
  • Therefore, our FV under low power is 4000 microns

11
Questions
  • Read pages 25-26, 171, 1070-1071 and answer the
  • following questions below
  • (A) Compare light (compound) microscopes to
  • electron microscopes.
  • (B) Compare and contrast
  • Transmission Electron Microscopes (TEM)
  • Scanning Electron Microscopes (SEM)
  • (C) Explain how to prepare a wet mount slide

12
TEM
13
SEM
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