Establishment of National Genetically Modified Product Detection Laboratories and Detection Method C

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Establishment of National Genetically Modified
Product Detection Laboratories and Detection
Method Complex

• ZHU Shui-fang, Ph.D
• GMOs detection center
• Institute of Animal and Plant Quarantine

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Research contents

• 1. Detection technology of GMOs
• Real-time PCR
• DNA Microarray
• Captured PCR-ELISA
• Quicktest strip
• 2. Publications for GMOs detection
• 12 SN standard protocols
• 8 GB standard protocols
• 4 for regulation and detection technology
• 3. Construction of laboratories for GMOs detection

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Detection technology of GMOs

• Real-time PCR
• DNA Microarray
• Captured PCR-ELISA
• Quicktest strip

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Real-time PCR

• Screening Kits
• Target genes CaMV 35S Promotor, Nos teminator
  , NptII, Bar, FMV promotor, Pat
• Reference gene 18S rRNA
• Quantitative Kits
• Roundup Ready soybean
• Bt176 Maize
• Event-specific detection kits
• GTS40-3-2, Bt176, Mon810, Bt11, GA21, T25, RT73

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Real time PCR - TaqMan Technology
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Positive sample
Negative sample
Ct value
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Screening Detection of GM potato and rapeseed
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Amplification of EPSPS gene
Amplification of lectin gene
Quantitative detection of Roundup Ready Soybean
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Real-time PCR kits
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DNA Microarray
Total 46 genes 31 inserted genes e.g.
EPSPS 7 reference genes e.g. lectin
8 event-specific genes e.g. Roundup Ready Soybean
Genes involved in microarray research
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DNA Microarray Technology
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Specific detection for RUR soybean
Multiple PCR and hybridization results of rapeseed
Multiple PCR and hybridization results of GM maize
Specific detection for Mon810 maize
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Specific detection for Bt11 maize
Multiple PCR and hybridization results of soybean
Specific detection for GA21 maize
Specific detection for Bt176 maize
Multiple PCR and hybridization results of cotton
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DNA Microarray kits
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Captured PCR-ELISA
Capture of target nucleic acid molecule PCR-ELISA
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PCR product
probe
electrophoresis
hybridization
PCR-ELISA Procedure
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Quicktest strip for Cry1Ab/Ac
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Publications for GMOs detection

• 4 for regulation and detection technology
• 12 SN standard protocols
• 8 GB standard protocols

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• GMOs regulation and detection technology
• 2 for regulation, 2 for detection
  technology
• SN and GB protocols for GMOs detection
• 12 SN (issued in 2003),
• 8 GB (will soon be issued)
• Detection methods (revising, ISO 7th
  Working group )
• Bioscien tomato, Mon531 cotton, RT73
  rapeseed, Edible Oil

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Publications of GMOs regulation and detection
technology
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12 SN standard protocols
The general requirements for the laboratories
for gene detection and identification Protocol
of the Qualitative Polymerase Chain Reaction
(PCR) for Detecting Genetically Modified
Component in Soybeans Protocol of the
Polymerase Chain Reaction for Detecting
Genetically Modified Components in Potatoes
Protocol of the Polymerase Chain Reaction for
Detecting Genetically Modified Components in
cotton Protocol of the real-time PCR for
detecting genetically modified plants and their
derived products Protocol of PCR for detection
of genetically modified feed Protocol of the
Polymerase Chain Reaction for Detecting
Genetically Modified Components in maize
Methods of sampling and preparation of samples
for detection of genetically modified components
in plants and their derived products Protocol
of the Detection Methods for Genetically Modified
tobacco Protocol of the polymerase chain
reaction for detecting genetically modified in
rapeseeds Protocol of the Qualitative
Polymerase Chain Reaction for Detecting
Genetically Modified Plant Components in Food
Protocol of the Qualitative Polymerase Chain
Reaction for Detecting Genetically Modified Plant
Components in Edible Oil
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8 GB standard protocols
Detection of genetically modified organisms and
derived products - Nucleic acid extraction
Detection of genetically modified organisms and
derived products Gene chip detection
Detection of genetically modified organisms and
derived products Protein based
methods Detection of genetically modified
organisms and derived products - Methods for
sampling and sample preparation Detection of
genetically modified organisms and derived
products - Quantitative nucleic acid based
methods Detection of genetically modified
organisms and derived products - Qualitative
nucleic acid based methods Detection of
genetically modified organisms and derived
products - General requirements for
laboratories Detection of genetically modified
organisms and derived products - General
requirements and definitions
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Construction of labs for GMOs detection

• 25 labs for GMOs detection
• Proficiency test
• Training of detection technology

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Thank you!