How Do the Antiseptics and Disinfectants We Use Everyday Work - PowerPoint PPT Presentation

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How Do the Antiseptics and Disinfectants We Use Everyday Work

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Gram staining both gram positive and gram negative ... Gram Staining ... Hans Christian Gram discovered that some stains are taken up by certain bacteria ... – PowerPoint PPT presentation

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Title: How Do the Antiseptics and Disinfectants We Use Everyday Work


1
How Do the Antiseptics and Disinfectants We Use
Everyday Work?
  • S.L.I.M.E. 2005
  • Kelly L. Shipley

2
Funding and support received from
3
Todays Agenda
  • Introduction
  • Lab Analysis of last class results
  • More information on Gram positive and Gram
    negative bacteria
  • One more basic technique the dally rod
  • Gram staining both gram positive and gram
    negative cultures
  • Anti-microbial testing
  • Closing

4
The Exciting Bacterial Cell
5
Gram and Gram- bacteria
  • Bacteria have cell walls for many reasons
  • 1) Withstand outside pressure
  • 2) Give shape to the cell
  • 3) Give rigidity to cell
  • These cell walls have many different components

6
Bacterial Cell Wall Components
7
Gram Positive Bacteria
  • Cytoplasmic membrane is a bilayer
  • 90 peptidoglycan
  • Low lipid content in cell wall
  • Teichoic Acids present

8
Gram Negative Bacteria
  • Cytoplasmic membrane is a bilayer
  • 10-20 peptidoglycan
  • Higher lipid content in cell wall
  • Multilayered Structure / Highly complicated

9
Gram Staining
  • 1884 Hans Christian Gram discovered that some
    stains are taken up by certain bacteria while
    others are not
  • Individual bacterial cells are hard to see,
    partly because they are small, but also because
    they are almost transparent.

10
Gram Staining
  • Gram Positive and Gram Negative Cell Walls are
    Very Different
  • Look at several different depictions of how they
    are so different

11
Gram Staining
  • Gram Positive and Gram Negative Cell Walls are
    Very DifferentAgain

12
Gram Staining Procedure
  • Gram Staining
  • Heat Fix the bacteria to the slide
  • Flood heat-fixed smear with crystal violet and
    let stand 30 seconds RINSE
  • Flood with Grams Iodine and let stand 60
    seconds RINSE
  • Holding slide at an angle, drop alcohol over
    smear until purple no longer runs off RINSE
  • Flood smear with safranin and let stand 30
    seconds, RINSE
  • Blot to dry, and focus through oil immersion

13
Gram Staining Procedure
14
Example - Gram Stained Slides
15
Anti-Microbial Testing
  • Goal To see how the products we use in our
    homes affect the different bacteria we know are
    around.
  • What types of products are out there?

16
Disinfectants
  • Disinfectants are substances that inhibit
    bacterial growth
  • THEY ARE ALSO HARMFUL TO HUMAN TISSUES!!

17
Disinfectants
  • Disinfectants are substances that inhibit
    bacterial growth
  • THEY ARE ALSO HARMFUL TO HUMAN TISSUES!!

18
Disinfectants
  • Disinfectants are substances that inhibit
    bacterial growth
  • THEY ARE ALSO HARMFUL TO HUMAN TISSUES!!

19
Antiseptics
  • Antiseptics are chemical substances that inhibit
    bacterial growth
  • THEY ARE NOT HARMFUL TO HUMAN TISSUES

20
Antiseptics
  • Antiseptics are chemical substances that inhibit
    bacterial growth
  • THEY ARE NOT HARMFUL TO HUMAN TISSUES

21
Antiseptics
  • Antiseptics are chemical substances that inhibit
    bacterial growth
  • THEY ARE NOT HARMFUL TO HUMAN TISSUES

22
What do you think?
  • Will antiseptics, which we use on our bodies, be
    more effective on Gram or Gram bacteria?
  • What about disinfectants?

23
Antibiotics
  • Antibiotics are also microorganisms, but we will
    not be talking about them in this class.

24
Lab InformationPay Attention
  • The following is an introduction to some of the
    materials we will be using today
  • Pay attention!!
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