Project 3: Ultrasound Biomicroscopy, Technology Development

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Project 3 Ultrasound Biomicroscopy,Technology
Development

• High frequency transducers and beamformers
• Guided injection
• Scanhead development
• Doppler and flow sensing
• High frequency nonlinear and contrast

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Commercial Partners
Collaborating Groups
MICe, Mt Sinai, Robarts, Queens, Univ of Virginia

• VisualSonics

Commercial Collaborators
AstraZeneca, Schering, Bracco, Imclone
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Transducers and Beamformers

• Marc Lucaks, Jian Hua Yin, Richard Garcia,
  Guofeng Pang, Kasia Harasiewicz, Jeremy Brown

Doppler / Flow
Andrew Needles, Victor Yang
Nonlinear / Contrast
Manu Cherin, Ross Williams, Olivier Couture,
Kevin Cheung
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300 microns
5 MHz
30 microns
50 MHz
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VisualSonics VEVO 660 Mouse Scanner

• B Mode (30 60) Hz
• M Mode
• Pulsed Doppler
• EKV
• 3D

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Realtime Scanhead
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Xenopus transverse section
UBM - 30 fps
1 mm
Yang, Needles et al U of Toronto
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Micro-injection into the Midbrain
30 frames/s, RMV Probe
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Chick embryo Day 3 Longitudinal Section
Gharib/Brigande/Sahn/Thornberg labs Cal Tech
and Oregon Health and Sciences University
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Chick embryo Day 3 Longitudinal Section
Gharib/Brigande/Sahn/Thornberg labs Cal Tech
and Oregon Health and Sciences University
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Array Development

• Improve depth of field
• Eliminate mechanical motion system
• Improve system reliability

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Annular Array Development
Jeremy Brown, Geoff Lockwood
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(No Transcript)
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Theoretical and Experimental Radiation Patterns
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FPGAs
A/Ds
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High Frequency Linear Array Development
300 microns
5 MHz
30 microns
50 MHz
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Array Structure
75 microns
SEM Images of Final Array Structure
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Inner Electrodes Bond pad Pitch 150 um Bond pad
opening 70 um
Vendor Gennum Material Alumina ceramic Metal
Layer Au-8,000 Angs. Indium Solder Indalloy
1E Dimension20 x 4 x 2 mils
1 mm
Solder Reflow
Richard Garcia and George Brown College
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Impedance of an Array Element
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Doppler / Flow Imaging

• Cardiogenesis in animal models
• Cardiovascular Hemodynamics
• Micro-circulation
• Tumour blood flow

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Imaging Tissue Motion SAD map

• Frame to frame clutter filtering with UBM at 15
  fps

?R
Yang et al U of Toronto
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Chick embryo Day 3 Longitudinal Section
Yang et al U of Toronto
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Doppler Colour Flow Imaging
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Ultrasound Molecular Imaging
Microbubble Contrast Agents
Nanoparticle Contrast Agents

• .5 5 microns
• Gas filled
• Inherently nonlinear
• explodable

• .1 1 microns
• Liquid filled
• Impedance enhanced signal

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Microbubble Contrast Agents
6 Million frames/s
Chin, deJong et al, Erasmus U Rotterdam
30 frames/s
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Albumin Microbubble Phagocytosis

• Denatured albumin in microbubble shell adheres to
  activated leukocytes attached to inflamed
  endothelium
• Albumin microbubbles remain acoustically active
  after phagocytosis

Courtesy of Dr. JR Lindner, Circulation 2000102
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30 min
Inflammation Targeted Microbubbles
2 h
Post-ischemia inflammation seen with leukocyte
targeted lipid microbubbles whose shell contains
phosphatidylserine
Infarct (TTC)
MB-PS
Christiansen JP, et al., Circulation 2002
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Submicron Particles

• Liquid perflurocarbon, water, surfactant

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Ultrasound enhancement caused by the addition of
submicron particles
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Targeted contrast enhancement for ultrasound
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Project 3 Future Developments

• Focus on array development
• Special emphasis on beamformer
• Improve sensitivity and quantitation for flow
  assessment
• Develop, characterize and validate targeted
  contrast agents