Enzyme Kinetics II

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Enzyme Kinetics II
DONT SHAKE THE ENZYME!!!!
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LT What Happened in the Test Tube Last Week?
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1 How fast did this happen?
Enzyme Kinetics
What environmental factors affect enzyme kinetics?
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2Last Week-Part A Calculating S using the
Lambert-Beer Plot

• S substrate concentration C
• C A / El A Absorbency _at_ 405 nm using
  Spec 20
  (get average /- SD across all
  groups)
• El, product of path length and extinction
  coefficient slope
• Determining El
  
  El A/C

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3 Last week- Part B Calculating V
S substrate concentration C
V C/ time C A / El A
Absorbency El product of path length and
extinction coefficient. 1) Use the El
calculated in part A to calculate C. 2) Knowing
C, Calculate V (C/time) 3) Knowing C and V,
determine Km and Vmax
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4Week2- Part A Effects of pH on Enzyme
Kinetics
pH Dependent Activation Most enzymes function in
a limited pH range. Cells use this a way to
control function
Relatively small changes in pH can greatly
affect rate of enzyme activity.
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5 This Week- Part B Effects of Temperature on
Enzyme Kinetics
Temperature Dependent Activation Enzymes are a
Fussy Bunch
Relatively small changes in human body temp can
greatly affect rate of enzyme activity.
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Its because of chemistry that homeostasis is
essential to our survival!
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6This Week- Part C Effect of Enzyme on v

• What do you think will happen?
• Form a hypothesis and test it using the protocol
  in C.
• Come up with a plan to graph your results!

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7Next Week What do YOU want to know about
Enzyme Kinetics?

• How do substances we put in our bodies affect
  enzyme kinetics?
• How do pollutants in our environment affect
  enzyme kinetics?
• What concentration ranges should you be
  considering?

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8 Group Power Point Presentations March 17th,
18th, 19th
Presentation 25pts 15pts power point
slides 10 pts presentation style

• Time Frame 12-15 min per group 5 min for
  questions
• Evaluation Dr. Hettes 85 , student input
  15
• Humor that adds to the Audiences understanding
  Good
• Humor that distracts from the audiences
  understanding Bad
• Power point slides must include graphs or tables
  of data for all
• 3 weeks, but you can spend your presentation
  time focused on your
• Design Your Own Experiment from week 3.