Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production

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Characterization of sugar-response Arabidopsis
(Arabidopsis thaliana) mutants to engineer plants
for higher ethanol, soydiesel and soy protein
production

• By Xin Li

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Purposes

• To find sugar-regulating genes that direct the
  flow of sugar to harvested portions of the plant.

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Purposes

• To find sugar-regulating genes that direct the
  flow of sugar to harvested portions of the plant.
• To produce cheaper soydiesel, more soy protein,
  and less expensive ethanol from the soybean
  plants.

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Modeling Soybean
Arabidopsis (Arabidopsis thaliana)
Soybean (Glycine max)
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Modeling Soybean
Arabidopsis (Arabidopsis thaliana)
Soybean (Glycine max)
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Modeling Soybean
Arabidopsis (Arabidopsis thaliana)
Soybean (Glycine max)
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Modeling Soybean

• Both are oilseed plants
• Arabidopsis has a mapped genome
• Insertion-induced Arabidopsis mutants are av
• ailable commercially

8
Modeling Soybean

• Both are oilseed plants
• Arabidopsis has a mapped genome
• Insertion-induced Arabidopsis mutants are av
• ailable commercially

9
Modeling Soybean

• Both are oilseed plants
• Arabidopsis has a mapped genome
• Insertion-induced Arabidopsis mutants are av
• ailable commercially

• Insertion-induced Arabidopsis mutants are
  available commercially

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Insertion-Induced Mutation
T-DNA
Gene
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Insertion-Induced Mutation
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Insertion-Induced Mutation
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Insertion-Induced Mutation
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Insertion-Induced Mutation
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Insertion-Induced Mutation
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(No Transcript)
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Wild-type
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Hypersensitive mutant
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Insensitive mutant
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Methods

• Grew 300 seedlings of each of 58 different
  mutants and wild-type in

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Methods

• Grew 300 seedlings of each of 58 different
  mutants and wild-type in
• 6 glucose

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Methods

• Grew 300 seedlings of each of 58 different
  mutants and wild-type in
• 6 glucose
• 6 sucrose

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Methods

• Grew 300 seedlings of each of 58 different
  mutants and wild-type in
• 6 glucose
• 6 sucrose
• Measured root length of seedlings grown in
  glucose (Gibson, 2005)

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Methods

• Grew 300 seedlings of each of 58 different
  mutants and wild-type in
• 6 glucose
• 6 sucrose
• Measured root length of seedlings grown in
  glucose (Gibson, 2005)
• Use spectrophotometry to determine anthocyanin
  levels of seedlings grown in sucrose (Nacry,1998)

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Results
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Results
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Results
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Results
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Results
Neff and Chory (1998)
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Results
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Conclusion

• I found a mutant that is hypersensitive to 6
  glucose and 6 sucrose.

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Conclusion

• I found a mutant that is hypersensitive to 6
  glucose and 6 sucrose.
• The mutants genotype is SALK_113292.

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Conclusion

• I found a mutant that is hypersensitive to 6
  glucose and 6 sucrose.
• The mutants genotype is SALK_113292.
• The disabled gene in mutant SALK_113292 is
  at1g06230.

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Conclusion

• I found a mutant that is hypersensitive to 6
  glucose and 6 sucrose.
• The mutants genotype is SALK_113292.
• The disabled gene in mutant SALK_113292 is
  at1g06230.
• The gene contains a bromodomain protein

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Future Work

• Grow SALK_113292 in 1 glucose and 1 sucrose

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Future Work

• Grow SALK_113292 in 1 glucose and 1 sucrose
• Grow SALK_113292 in 1 sorbitol and 6 sorbitol

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Image from htto//www.food-info.net
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Acknowledgement

• Dr. Sue Gibson
• Dr. Chunyao Li
• Ms. Lois Fruen
• Ms. Chelen Johnson
• Dr. Jacob Miller
• Ms. Virginia Amundson
• Breck Advanced Team Research

http//www.cbs.umn.edu/plantbio/faculty/GibsonSue/
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Characterization of sugar-response Arabidopsis
(Arabidopsis thaliana) mutants to engineer plants
for higher ethanol, soydiesel and soy protein
production

• By Xin Li