Update of Mosquitoes, Disease Surveillance, and Susceptibility Testing in Jacksonville - PowerPoint PPT Presentation

Loading...

PPT – Update of Mosquitoes, Disease Surveillance, and Susceptibility Testing in Jacksonville PowerPoint presentation | free to view - id: 10f65e-ZDc1Z



Loading


The Adobe Flash plugin is needed to view this content

Get the plugin now

View by Category
About This Presentation
Title:

Update of Mosquitoes, Disease Surveillance, and Susceptibility Testing in Jacksonville

Description:

Marah Clark, Entomologist. Jacksonville Mosquito Control. Arboviral Update ... Dibrom has been applied by aircraft for over 30 years in Duval County ... – PowerPoint PPT presentation

Number of Views:47
Avg rating:3.0/5.0
Slides: 17
Provided by: mcl129
Category:

less

Write a Comment
User Comments (0)
Transcript and Presenter's Notes

Title: Update of Mosquitoes, Disease Surveillance, and Susceptibility Testing in Jacksonville


1
Update of Mosquitoes, Disease Surveillance, and
Susceptibility Testing in Jacksonville
  • Marah Clark, Entomologist
  • Jacksonville Mosquito Control

2
Arboviral Update
  • 66,500 mosquitoes collected from NJLT in 2007
  • Seroconversions
  • Human Cases 0
  • Sentinel 2, 1 EEE and 1 WN at same site
  • Horses 0
  • Dead Birds 0
  • Mosquito pools 0

3
Bottle Bioassay in Duval County
  • Testing Laboratory Resistance of
  • Culex quinquefasciatus to Dibrom

4
Objective
  • Dibrom has been applied by aircraft for over 30
    years in Duval County
  • Culex quinquefasciatus is a vector species
  • Evidence suggests that aerial adulticiding is not
    efficacious in controlling Culex in Jacksonville
    (2004 CDC collections after aerial spray
    application)
  • Is there any significant resistance by wild Culex
    quinquefasciatus to Dibrom?

5
Procedure
  • Review bottle bioassay protocol, including
    documentation on preparing Dibrom test solution.
  • www.pherec.org/memoranda/procedure.html
  • Collect and rear native mosquitoes
  • Collected in larval stage from select catch
    basins
  • Reared using artesian well water and pulverized
    dog food
  • Obtain Dibrom sample from chemical stored on site

6
Appropriate Test Solution Concentration
  • Diagnostic concentration of 25 ug/mL Naled
  • 1mL of test solution used to prepare 4 bottles
    for each test
  • 1mL of ethanol used to prepare 2 control bottles
    for each test
  • Mosquitoes aspirated and introduced to bottles
  • Number of dead mosquitoes recorded every 15
    minutes, until 100 mortality obtained

7
Percent Mortality, May 2007
8
(No Transcript)
9
(No Transcript)
10
Percent Mortality, December 2007
11
(No Transcript)
12
(No Transcript)
13
(No Transcript)
14
Conclusions
  • Culex quinquefasciatus collected in Jacksonville
    displayed uniform susceptibility to Dibrom
    concentrate
  • Consistency of how the test is conducted is
    important to maintain statewide standards
  • County continues to see uniform susceptibility,
    though further testing is warranted

15
Next Question
  • If resistance is not a problem, is correct
    concentration being applied for the area?
  • What may be other causes that would prevent
    effective control?
  • Mosquito behavior minimizes vulnerability
  • Weather (humidity, wind, temp., etc.)
  • Mission timing (dusk versus dawn)
  • Aerosol equipment, technique

16
Notes/Future Tests
  • Bottle Bioassay results indicate resistance only.
    They will not point toward appropriate field
    application rates.
  • Bottle concentration is 2 X LC95 and is designed
    to create a kill situation.
  • Plans to run pre- and post- season tests for
    resistance in Culex species to Dibrom
  • Run permethrin tests for Culex species pre- and
    post- season
  • Attempt to conduct a Dose Response Curve using
    Log Dose Probit (LDP) to infer appropriate
    concentrations for both Cx. nigripalpus and Cx.
    quinquefasciatus
About PowerShow.com