Mammalian Cell Expression - PowerPoint PPT Presentation

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Mammalian Cell Expression

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Profacgen provides you with an ultimate solution of mammalian protein production. Our service can be tailored with your specialized requirements. Our expertise guarantees high protein quality, short turnaround time, and the best price in the market! – PowerPoint PPT presentation

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Title: Mammalian Cell Expression


1
Mammalian Cell Expression
  • Profacgen

2
  • Mammalian cell expression systems are the best
    choice for the production of eukaryotic proteins,
    especially when correct folding and
    post-translational modification (glycosylation,
    phosphorylation, etc.) is required. They produce
    eukaryotic recombinant proteins in the most
    natural state, with native tertiary structure,
    physiochemical characteristics, and
    bioactivities. 

3
  • They have been successfully applied in the
    biopharmaceutical production of cytokines,
    monoclonal antibodies, growth factors and so on.
    The most widely-used mammalian cell lines are
    HEK293 and CHO cells.

4
  • Features
  •  Protein production with accurate
    post-translational modification Bioactive
    protein production Transient and stable
    expression Large-scale protein production

5
HEK293 expression
  • Human Embryonal Kidney 293 (HEK293) cells have
    been extensively used for the transient
    expression of recombinant proteins. Their ease of
    culture and highly efficient transfection rate
    make them especially suitable for customized
    protein production for research use.

6
CHO expression
  • Chinese Hamster Ovary (CHO) cells, which have
    been successfully used for the manufacture of
    FDA-approved therapeutic proteins and antibodies,
    are prominent in stable expression. Stably
    transfected CHO cell line is the most suitable
    mammalian host for high-yield production of
    recombinant proteins. They are adaptable to
    diverse culture conditions. Protein purification
    from CHO culture is also relatively easy because
    of their secretory feature.

7
  • Profacgen provides you with an ultimate solution
    of mammalian protein production. Our service can
    be tailored with your specialized requirements.
    Our expertise guarantees high protein quality,
    short turnaround time, and the best price in the
    market!

8
  • Thanks for watching!
  • Contact Profacgen
  • Address 45-1 Ramsey Road Shirley, NY 11967, USA
  • Website https//www.profacgen.com
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