Introduction for Absolute Quantification - PowerPoint PPT Presentation

About This Presentation
Title:

Introduction for Absolute Quantification

Description:

this is the basic introduction to the absolute quantification – PowerPoint PPT presentation

Number of Views:6
Slides: 3
Provided by: susanrey

less

Transcript and Presenter's Notes

Title: Introduction for Absolute Quantification


1
Introduction for Absolute Quantification
2
Introduction for Absolute Quantification
  • Summary Absolute Quantification, a technique, is
    a targeted quantitative proteomics. This kind of
    technique can express the strong efficacy and is
    being increasingly used for a full range of
    quantitative proteomics research. We will mainly
    introduce the function and advantages of
    technique--Absolute Quantification.
  •  
  • Body The technique AQUA method can not only be
    the proteins absolute quantification( AQUA), but
    their modification states. Peptides are combined
    with incorporated stable isotopes, which is
    regarded as the ideal internal standards for the
    mimic native peptides, and this native peptides
    is formed by proteolysis. We also can prepare
    these synthetic peptides by using covalent
    modifications, such as methylation, acelylation,
    phosphorylation. The modification we used are
    identical chemically to naturally happening
    posttranslational modifications. After that, this
    kind of AQUA internal standard peptides are
    utilized to exactly and quantitatively measure
    the absolute levels of proteins and
    post-translationally changed proteins after
    proteolysis with the method of a selected
    reaction monitoring analysis, which happens in a
    tandem mass spectrometer.
  •  
  • The development in biological mass spectrometry
    have made the advances of numerous methods for
    the large-scale quantification of protein
    expression levels, which all are processing in
    the cells. In addition to the measurements of
    protein expression accomplished by differential
    incorporation of stable isotopes in to cellular
    proteins. for the proteomics analysis, the
    absolute quantification is also an wonderful way.
  •  
  • The functions of the absolute quantification a
    common process for the post-translational
    modification and the quantification of proteins.
    Absolute quantification can be offered by AQUA
    with employment of synethic peptides, which is
    mixed with stable isotope.
  •  
  • There are one base for the absolute
    quantification method, it is the discovery of
    unexpected relationship between the protein
    concentration and MS signal response. The average
    MS signal response, which is for the three most
    intense tryptic peptides per mole of protein, is
    not going to be changed within a coefficient if
    variation of less than 10. This relationship can
    be utilized to estimate a general signal response
    factor. If given an internal standard. The
    general signal response factor was expressed to
    be similar to all protein testing. However the
    isotope way build only relative quantification of
    proteins, which are expressed. The absolute
    quantification(AQUA) method can offer for precise
    determination of protein expression and the
    levels of post-translational modification. This
    AQUA method depends on the use of a synthetic
    internal level peptide, which is introduced
    during digestion, as a known concentration to the
    cell lysates. When we analyze the proteolyzed
    with a selected reaction monitoring(SRM)
    experiment, which is processed in a tandem mass
    spectrometer. It is may cause the direct
    detection and the native peptides quantification
    and isotope, which is labeled with AQUA internal
    standard peptide.
  •  
  • Because of the sensitivity and simplicity of the
    method, added with the tandem mass spectrometers
    widespread availability, AQUA method becomes a
    most useful process for us to measure the
    proteins levels and post-translational
    modifications directly from cell lysates.
  •  
  • About the author Creative Proteomcis, the
    proteomcis division of CD Inc, has the whole
    services in the drug development field. We are
    specializing in the hard-to-analyze samples, that
    is because of the scientists, who have many
    experience in this field. We also have maintained
    the close relationship with our partners,
    providing the lowest cost for these industry.
  •  
  • Links
  • http//www.creative-proteomics.com/Services/Absolu
    te-quantification-AQUA.htm
  •  
  • http//www.creative-proteomcis.com
  •  
Write a Comment
User Comments (0)
About PowerShow.com