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The aim is to provide a design for modular setup, integrating Correlation Electrophoresis and Vacanc

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( VAC) Rinse reference or current sample from C to D ... Trace Quality control (COR VAC) Rinse reference sample C to D, then A to B. Rinse sample A to C ... – PowerPoint PPT presentation

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Title: The aim is to provide a design for modular setup, integrating Correlation Electrophoresis and Vacanc


1

discussion poster Jetse Reijenga
Setup for Correlation Vacancy Electrophoresis in
trace analysis, process monitoring, finger
printing, trend analysis
Some design parameters All vessels have
vacuum/pressure for rinsing. Negative HV (because
of EOF) is on detection side, to enable easier
isolation of sample vessels (required). Separate
design for time-shifted sampling. Some
operating parameters Integration time, duty
cycle, minimum and maximum sampling length of
PRBS in COR mode (depends on sample
load) Classic mode of operation Rinse BGE from
C to D, then sample from A to B Electrophorize
from C to D, classis pherogram Trace analysis
mode (COR) Rinse BGE from C to D, then A to B,
then sample from A to C Electrophorize from A to
D and from B to D programmed by PRBS, then
cross-correlate signal with PRBS Quality
control or trend anal. (VAC) Rinse reference or
current sample from C to D Rinse sample or
time-shifted sample from A to B Electrophorize
from C to D, givers differential
pherogram Trace Quality control
(CORVAC) Rinse reference sample C to D, then A
to B Rinse sample A to C Analyse A to D and B
to D programmed by PRBS, then cross correlate
signal with PRBS Trace trend analysis
(CORVAC) Rinse reference sample C to D, then A
to B Rinse time-shifted sample A to C Analyse A
to D and B to D programmed by PRBS Cross-correlat
e signal with PRBS Bottle necks Some of the
potential bottle necks of the proposed
schemes On the long run, despite measures
taken, EOF drift may occur. On a shorter term,
EOF shift will lead to peak broadening, in the
long run, to peak shifting For operation in VAC
mode, ghost-peaking 5 may occur Voltage
switching leads to spikes in detector signal.
Introduction The aim is to provide a design for
modular setup, integrating Correlation
Electrophoresis and Vacancy (a.k.a. Differential)
Electrophoresis. A block diagram and construction
guidelines are given and discussed and the
working procedures for different modes of
operation are outlined. Targeted fields of
application are trace analysis, finger printing,
process monitoring and trend analysis. Correlati
on Electrophoresis Correlation- or mulitplex-,
or multiple injection chromatography was
introduced for GC and LC by Smit et al 1.
Kuldvee et al also applied the principle to
Electrophoresis 2. Sample (single injection in
A) and BGE are injected alternately during the
whole detection period, using a file of Pseudo
Randomized Binary Sequence (PRBS, 3) (B). The
resulting detection signal (not shown) is then
cross-correlated with that same file (B), to
obtain a signal with improved S/N ratio
(C). A B C
Vacancy (differential) Electrophoresis Vacancy
(or differential) Electrophoresis was developed
by Mikkers 4. BGE is injected in a separation
chamber filled with sample. A negative
electropherogram of the sample composition is
obtained. Alternatively, a differential
electropherogram C of 2 sample solutions S1 and
S2 can be obtained. S1 S2
S1 in S2
Building blocks A CE separation chip is to be
used, which has 3 entrance (A, B and C) and 1
exit (D) connection to perpendicularly mounted
channels, and an optical on-chip detector.
Conclusion or ???
References 1. H.C. Smit, Chromatographia 3 (1970)
515 2. R. Kuldvee et al, J. High Resol.
Chromatogr. 21(1998) 169 3. M. Kaljurand, E.
Küllik, Computerised Multiple Input
Chromatography, Ellis Horwood Ltd., Chichester
(1989) 4. F.E.P. Mikkers, Anal. Chem. 69 (1997)
333 5. J.L. Beckers, P. Bocek, Electrophoresis 20
(1999) 519
ISSS-2005
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